Human Reproduction, Vol. 16, No. 6, 1172-1179,
June 2001
© 2001 European Society of Human Reproduction and Embryology
Aneuploidy rate in spermatozoa of selected men with abnormal semen parameters*
1 Division of Endocrinology and Master in Andrological Sciences: New Methodologies in Human Reproductive Medicine, University of Catania, 2 Master in Endocrinological and Metabolic Sciences, University of Naples, Naples, 3 Department of Human Anatomy and 4 Department of Animal Biology, University of Catania, Italy
A large proportion of patients with oligoasthenoteratozoospermia (OAT) have an abnormal karyotype and hence they produce aneuploid gametes. However, a normal karyotype does not exclude the chance of having germ cell aneuploidy, since an altered intra-testicular environment not only damages spermatogenesis, but may also disrupt the mechanisms controlling chromosomal segregation during meiosis. Therefore, this study was undertaken to evaluate the rate of aneuploidy in the spermatozoa of selected patients with abnormal sperm parameters. For this purpose, sperm aneuploidy rate for chromosomes 8, 12, 18, X and Y was evaluated by multicolour fluorescence in-situ hybridization (FISH) in nine patients with teratozoospermia alone and 19 OAT patients of presumably testicular origin. Thirteen normozoospermic healthy men served as controls. Patients with teratozoospermia or OAT had significantly greater disomy and diploidy rates compared with controls, whereas the rate of nullisomy was similar. XY disomy was very low in all groups, suggesting that chromosomal non-disjunction occurs mainly during the second meiotic division. Autosome 12 disomy rate was low in both patients and controls. There was a marked variability of total sperm aneuploidy rate in both groups of patients. Sperm aneuploidy rate was negatively correlated with sperm concentration and particularly with the percentage of normal forms. In conclusion, patients with teratozoospermia or OAT have an increased rate of sperm aneuploidy. This increase is similar in both groups, suggesting that teratozoospermia may be the critical sperm parameter associated with aneuploidy.
Key words: chromosomes 8, 12 and 18/multicolour fluorescence in-situ hybridization/oligoasthenoteratozoospermia/sex chromosomes/sperm aneuploidy
5 To whom correspondence should be addressed at: Istituto di Medicina Interna e Specialità Internistiche, Cattedra di Endocrinologia, Ospedale Garibaldi, Piazza S. Maria di Gesù, 951213 Catania, Italy. E-mail: acaloger{at}unict.it
* Presented at the 16th Meeting of the European Society of Human Reproduction and Embryology, Bologna, Italy, June 25–28, 2000.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  M. Sanchez-Castro, A.R. Jimenez-Macedo, M. Sandalinas, and J. Blanco Prognostic value of sperm fluorescence in situ hybridization analysis over PGD Hum. Reprod., June 1, 2009; 24(6): 1516 - 1521. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Cavallini, A. Crippa, M. C. Magli, N. Cavallini, A. P. Ferraretti, and L. Gianaroli A Study to Sustain the Hypothesis of the Multiple Genesis of Oligoasthenoteratospermia in Human Idiopathic Infertile Males Biol Reprod, October 1, 2008; 79(4): 667 - 673. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Kirkpatrick, K. A. Ferguson, H. Gao, S. Tang, V. Chow, B. H. Yuen, and S. Ma A comparison of sperm aneuploidy rates between infertile men with normal and abnormal karyotypes Hum. Reprod., July 1, 2008; 23(7): 1679 - 1683. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. C. Wong, K. A Ferguson, V. Chow, and S. Ma Sperm aneuploidy and meiotic sex chromosome configurations in an infertile XYY male Hum. Reprod., February 1, 2008; 23(2): 374 - 378. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Prisant, D. Escalier, J-C. Soufir, M. Morillon, D. Schoevaert, M. Misrahi, and G. Tachdjian Ultrastructural nuclear defects and increased chromosome aneuploidies in spermatozoa with elongated heads Hum. Reprod., April 1, 2007; 22(4): 1052 - 1059. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Strassburger, M. Reichart, S. Kaufman, E. Kasterstein, D. Komarovsky, O. Bern, S. Friedler, M. Schachter, R. Ron-El, and A. Raziel Morphology assessment and fluorescence in situ hybridization of the same spermatozoon using a computerized cell-scanning system Hum. Reprod., January 1, 2007; 22(1): 201 - 209. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Mateu, L. Rodrigo, N. Prados, M. Gil-Salom, J. Remohi, A. Pellicer, and C. Rubio High Incidence of Chromosomal Abnormalities in Large-Headed and Multiple-Tailed Spermatozoa J Androl, January 1, 2006; 27(1): 6 - 10. [Full Text] [PDF]
|
|
|
|
|
|
L. Gianaroli, M.C. Magli, G. Cavallini, A. Crippa, M. Nadalini, L. Bernardini, G. F. Menchini Fabris, S. Voliani, and A. P. Ferraretti Frequency of aneuploidy in sperm from patients with extremely severe male factor infertility Hum. Reprod., August 1, 2005; 20(8): 2140 - 2152. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.R. Guichaoua, J. Perrin, C. Metzler-Guillemain, J. Saias-Magnan, R. Giorgi, and J.M. Grillo Meiotic anomalies in infertile men with severe spermatogenic defects Hum. Reprod., July 1, 2005; 20(7): 1897 - 1902. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-G. Pang, Y. J. Kim, S.-H. Lee, and C.-K. Kim The high incidence of meiotic errors increases with decreased sperm count in severe male factor infertilities Hum. Reprod., June 1, 2005; 20(6): 1688 - 1694. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. M. Petit, N. Frydman, M. Benkhalifa, A. Le Du, A. Aboura, R. Fanchin, R. Frydman, and G. Tachdjian Could Sperm Aneuploidy Rate Determination Be Used as a Predictive Test Before Intracytoplasmic Sperm Injection? J Androl, March 1, 2005; 26(2): 235 - 241. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Morel, N. Douet-Guilbert, A. Moerman, B. Duban, C. Marchetti, B. Delobel, M.-J. Le Bris, V. Amice, and M. De Braekeleer Chromosome aneuploidy in the spermatozoa of two men with globozoospermia Mol. Hum. Reprod., November 1, 2004; 10(11): 835 - 838. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Burrello, G. Arcidiacono, E. Vicari, P. Asero, D. Di Benedetto, A. De Palma, R. Romeo, R. D'Agata, and A. E. Calogero Morphologically normal spermatozoa of patients with secretory oligo-astheno-teratozoospermia have an increased aneuploidy rate Hum. Reprod., October 1, 2004; 19(10): 2298 - 2302. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Celik-Ozenci, A. Jakab, T. Kovacs, J. Catalanotti, R. Demir, P. Bray-Ward, D. Ward, and G. Huszar Sperm selection for ICSI: shape properties do notpredict the absence or presence of numericalchromosomal aberrations Hum. Reprod., September 1, 2004; 19(9): 2052 - 2059. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Rodrigo, C. Rubio, E. Mateu, C. Simon, J. Remohi, A. Pellicer, and M. Gil-Salom Analysis of chromosomal abnormalities in testicular and epididymal spermatozoa from azoospermic ICSI patients by fluorescence in-situ hybridization Hum. Reprod., January 1, 2004; 19(1): 118 - 123. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.S. Tang, H. Gao, W.P. Robinson, B. Ho Yuen, and S. Ma An association between sex chromosomal aneuploidy in sperm and an abortus with 45,X of paternal origin: possible transmission of chromosomal abnormalities through ICSI Hum. Reprod., January 1, 2004; 19(1): 147 - 151. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Celik-Ozenci, J. Catalanotti, A. Jakab, C. Aksu, D. Ward, P. Bray-Ward, R. Demir, and G. Huszar Human Sperm Maintain Their Shape Following Decondensation and Denaturation for Fluorescent In Situ Hybridization: Shape Analysis and Objective Morphometry Biol Reprod, October 1, 2003; 69(4): 1347 - 1355. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. H. Martin, A. W. Rademaker, C. Greene, E. Ko, T. Hoang, L. Barclay, and J. Chernos A Comparison of the Frequency of Sperm Chromosome Abnormalities in Men with Mild, Moderate, and Severe Oligozoospermia Biol Reprod, August 1, 2003; 69(2): 535 - 539. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Burrello, E. Vicari, P. Shin, A. Agarwal, A. De Palma, C. Grazioso, R. D'Agata, and A. E. Calogero Lower sperm aneuploidy frequency is associated with high pregnancy rates in ICSI programmes Hum. Reprod., July 1, 2003; 18(7): 1371 - 1376. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Vicari, A. De Palma, N. Burrello, G. Longo, C. Grazioso, N. Barone, M. Zahi, R. D'agata, and A. E. Calogero Absolute Polymorphic Teratozoospermia in Patients With Oligo-Asthenozoospermia Is Associated With an Elevated Sperm Aneuploidy Rate J Androl, July 1, 2003; 24(4): 598 - 603. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D.K. Griffin, P. Hyland, H.G. Tempest, and S.T. Homa Safety issues in assisted reproduction technology: Should men undergoing ICSI be screened for chromosome abnormalities in their sperm? Hum. Reprod., February 1, 2003; 18(2): 229 - 235. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Bonduelle, E. Van Assche, H. Joris, K. Keymolen, P. Devroey, A. Van Steirteghem, and I. Liebaers Prenatal testing in ICSI pregnancies: incidence of chromosomal anomalies in 1586 karyotypes and relation to sperm parameters Hum. Reprod., October 1, 2002; 17(10): 2600 - 2614. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Mateizel, G. Verheyen, E. Van Assche, H. Tournaye, I. Liebaers, and A. Van Steirteghem FISH analysis of chromosome X, Y and 18 abnormalities in testicular sperm from azoospermic patients Hum. Reprod., September 1, 2002; 17(9): 2249 - 2257. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.M. Luetjens, C. Rolf, P. Gassner, J.E. Werny, and E. Nieschlag Sperm aneuploidy rates in younger and older men Hum. Reprod., July 1, 2002; 17(7): 1826 - 1832. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Egozcue, J. Blanco, F. Vidal, and J. Egozcue Diploid sperm and the origin of triploidy Hum. Reprod., January 1, 2002; 17(1): 5 - 7. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. E. Calogero, A. De Palma, C. Grazioso, N. Barone, N. Burrello, I. Palermo, A. Gulisano, C. Pafumi, and R. D'Agata High sperm aneuploidy rate in unselected infertile patients and its relationship with intracytoplasmic sperm injection outcome Hum. Reprod., July 1, 2001; 16(7): 1433 - 1439. [Abstract] [Full Text] [PDF]
|
|