Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF ) Freely available
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (7)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Chen, C.-D.
Right arrow Articles by Yang, Y.-S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chen, C.-D.
Right arrow Articles by Yang, Y.-S.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Human Reproduction, Vol. 17, No. 1, 128-133, January 2002
© 2002 European Society of Human Reproduction and Embryology

The significance of cytokines, chemical composition, and murine embryo development in hydrosalpinx fluid for predicting the IVF outcome in women with hydrosalpinx

Chin-Der Chen, Jehn-Hsiahn Yang, Ko-Chen Lin, Kuang-Han Chao, Hong-Nerng Ho and Yu-Shih Yang,1

Department of Obstetrics and Gynecology, National Taiwan University College of Medicine and Hospital, Taipei, Taiwan

BACKGROUND: The aim of this study was to determine whether differences in cytokine concentrations, chemical composition, and murine embryogenesis in hydrosalpinx fluid could be observed between women with hydrosalpinx who achieved and did not achieve pregnancy. METHODS: Hydrosalpinx fluids were collected from 39 infertile women participating in an IVF–embryo transfer programme at a university teaching hospital. The fluids were analysed for concentrations of cytokine concentration (epidermal growth factor, interferon-{gamma}, leukaemia inhibitory factor, and tumour necrosis factor-{alpha}) and chemical composition. Mouse embryos were cultured in medium with various concentrations (0, 10, 50, 100%) of hydrosalpinx fluid. RESULTS: Logistic regression demonstrated that a murine blastocyst formation rate 53.3% in 50% hydrosalpinx fluid (OR = 16.6, 95% CI 2.4–116.1, P = 0.005) and patient age (OR = 0.778, 95% CI 0.61–0.99, P = 0.045) were independent predictors of IVF outcome. The diagnostic accuracy did not significantly improve when age and number of good quality embryos transferred was taken into account. The chemical composition and cytokine concentrations of hydrosalpinx fluid were not predictive of subsequent IVF outcome. CONCLUSIONS: The mouse embryo assay of hydrosalpinx fluid might potentially serve as a predictor of subsequent IVF outcome in women with hydrosalpinx. This technique may be useful in selecting appropriate treatment options for patients with hydrosalpinx undergoing IVF treatment.

Key words: cytokine/hydrosalpinx fluid/IVF outcome/mouse embryo assay

1 To whom correspondence should be addressed at: Department of Obstetrics and Gynecology, National Taiwan University Hospital, 7, Chung-Shan South Road, Taipei, Taiwan. E-mail: yayang{at}ntumc.org


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Hum ReprodHome page
L. C. Ajonuma, L. N. Chan, E. H. Y. Ng, P. H. Chow, L. S. Kung, A. N. Y. Cheung, C. Briton-Jones, I. H. Lok, C. J. Haines, and H. C. Chan
Characterization of epithelial cell culture from human hydrosalpinges and effects of its conditioned medium on embryo development and sperm motility
Hum. Reprod., February 1, 2003; 18(2): 291 - 298.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
A. Strandell and A. Lindhard
Why does hydrosalpinx reduce fertility?: The importance of hydrosalpinx fluid
Hum. Reprod., May 1, 2002; 17(5): 1141 - 1145.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.