Human Reproduction, Vol. 17, No. 12, 3039-3045,
December 2002
© 2002 European Society of Human Reproduction and Embryology
Birth of offspring following transplantation of cryopreserved immature testicular pieces and in-vitro microinsemination
1 Department of Medical Chemistry, Graduate School of Medicine, Kyoto University, Kyoto, 2 The Institute of Physical and Chemical Research (RIKEN), Bioresource Center, Ibaraki, 3 Laboratory of Reproductive Engineering, 6 Meiji University, Kawasaki, 4 Department of Pathology and Biology of Diseases, and 5 Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University, Kyoto, Japan
BACKGROUND: Fertility protection is an urgent clinical problem for prepubertal male oncology patients who undergo either chemotherapy or radiotherapy. As these patients do not have mature sperm to be frozen, there is as yet no effective method to preserve their fertility. METHODS AND RESULTS: Single pieces of immature mouse (1.5x1.5x1.5 mm) or rabbit (2.0x2.0x
3.0 mm) testis were cryopreserved, thawed and transplanted into mouse testes. Histological techniques were used to determine the presence of spermatogenesis, which was restored in both mouse and rabbit testicular pieces, and led to the production of mature sperm after both cryopreservation and syngeneic or xenogeneic transplantation into mouse testes. Using sperm developed in the frozen–thawed transplants, mouse offspring were born after in-vitro microinsemination. Furthermore, rabbit offspring were obtained using rabbit sperm that developed in fresh transplants in a xenogeneic surrogate mouse. CONCLUSIONS: This approach of ‘testicular tissue banking’ is a promising technique for the preservation of fertility in prepubertal male oncology patients. Xenogeneic transplantation into immunodeficient mice may provide a system for studying spermatogenic failure in infertile men.
Key words: cancer/infertility/in-vitro microinsemination/testis/transplantation
6 To whom correspondence should be addressed at: Department of Medical Chemistry, Graduate School of Medicine, Kyoto University, Kyoto, Japan, 606-8501; E-mail: takashi{at}mfour.med.kyoto-u.ac.jp
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