Human Reproduction, Vol. 17, No. 3, 601-604,
March 2002
© 2002 European Society of Human Reproduction and Embryology
Relationship between oxidative stress and embryotoxicity of hydrosalpingeal fluid
Presented at the 56th Annual Meeting of the American Society for Reproductive Medicine, San Diego, CA, October 21-25, 2000.
1 Center for Advanced Research in Human Reproduction and Infertility, Department of Gynecology & Obstetrics and 2 Department of Biostatistics and Epidemiology, The Cleveland Clinic Foundation, Cleveland, OH, USA
BACKGROUND: Oxidative stress mechanisms are involved in the pathophysiology of many reproductive disorders. The objective of this study was to characterize oxidative stress parameters in hydrosalpingeal fluid (HSF) and examine their possible role in early embryo development. METHODS AND RESULTS: HSF was aspirated at laparoscopic salpingectomy in 11 infertile women. Reactive oxygen species (ROS), total (non-enzymatic) antioxidant capacity (TAC) and lipid peroxidation (LPO) were assayed. Two-cell mouse embryos were incubated with 25, 50 or 75% HSF and the blastocyst development rate was observed. ROS was detected in five of 11 (45%) HSF samples with a mean of 4.2x104 c.p.m. LPO was detected in all samples at a mean (±SD) value of 5575.4 ± 6091.9 µmol/l malonaldehyde. The mean blastocyst development rate at 25, 50 and 75% HSF and in the control group was 88.9 ± 9.4, 65.7 ± 19.1, 45.7 ± 5.7 and 96.7% respectively (P < 0.0001). The blastocyst development rate was positively correlated to ROS concentrations (P < 0.02) but was not significantly related to LPO. CONCLUSIONS: The blastocyst development rate decreased with increasing concentrations of HSF. For the first time, the presence of ROS, LPO and TAC activity in human HSF was characterized. A possible role of oxidative stress in the embryotoxicity of HSF is suggested.
Key words: embryotoxicity/hydrosalpinx/lipid peroxidation/reactive oxygen species/total antioxidant capacity
3 To whom correspondence should be addressed at: The Cleveland Clinic Foundation, A19.1, 9500 Euclid Avenue, Cleveland, OH 44195, USA. E-mail: sharmar{at}ccf.org
Submitted on June 11, 2001; resubmitted on September 20, 2001
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