Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF ) Freely available
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (17)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Isobe, T.
Right arrow Articles by Toyoda, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Isobe, T.
Right arrow Articles by Toyoda, N.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Human Reproduction, Vol. 17, No. 6, 1441-1446, June 2002
© 2002 European Society of Human Reproduction and Embryology

The effect of RANTES on human sperm chemotaxis

Tetsuya Isobe1, Hiroyuki Minoura1,3, Keisuke Tanaka1, Takashi Shibahara2, Naoko Hayashi2 and Nagayasu Toyoda1

1 Department of Obstetrics and Gynecology, School of Medicine, Mie University, Edobashi 2-174, Tsu, Mie, Japan and 2 Suzuka Kaisei General Hospital, Kouchou Hoshizaka 112-1, Suzuka, Japan

BACKGROUND: Follicular fluid (FF) is a powerful stimulator of human sperm hyperactivation and the acrosome reaction. FF causes sperm accumulation by way of chemotaxis. The chemoattractant in FF has not yet been identified in the human. It is well known that some types of chemokine such as RANTES (Regulated on Activation Normal T Expressed and Secreted Chemokine) exist in genital tract fluids (for example, FF, seminal plasma and uterine fluid). Few reports appear to exist concerning the direct effect of chemokines on the function of human sperm. METHODS: The chemotactic effect of RANTES on human sperm were investigated by capillary and double-chamber assays, while the chemokinetic effect was investigated using computer-assisted sperm analysis. RESULTS: Both the capillary and double-chamber assays demonstrated a significant chemotactic effect of RANTES on human sperm. Anti-RANTES rabbit IgG partially neutralized the chemotactic effect of FF. In contrast, no statistically significant chemokinetic effect was observed in any motility parameter. CONCLUSIONS: Here, it was demonstrated that mRNA for the RANTES receptors CCR-1 and CCR-5 are present in human sperm. Furthermore, RANTES is involved in the chemotactic effect of FF in vitro.

Key words: chemotaxis/cytokine/follicular fluid/human sperm/RANTES

3 To whom correspondence should be addressed at: Department of Obstetrics and Gynecology, School of Medicine, Mie University, 514-8507, Edobashi 2-174, Tsu, Mie, Japan. E-mail: hminoura{at}clin.medic.mie-u.ac.jp


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Hum Reprod UpdateHome page
S. S. Suarez
Control of hyperactivation in sperm
Hum. Reprod. Update, July 24, 2008; (2008) dmn029v1.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
A. Barbonetti, M.R.C. Vassallo, C. Antonangelo, V. Nuccetelli, A. D'Angeli, F. Pelliccione, M. Giorgi, F. Francavilla, and S. Francavilla
RANTES and human sperm fertilizing ability: effect on acrosome reaction and sperm/oocyte fusion
Mol. Hum. Reprod., July 1, 2008; 14(7): 387 - 391.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
M. Shimada, Y. Yanai, T. Okazaki, N. Noma, I. Kawashima, T. Mori, and J. S. Richards
Hyaluronan fragments generated by sperm-secreted hyaluronidase stimulate cytokine/chemokine production via the TLR2 and TLR4 pathway in cumulus cells of ovulated COCs, which may enhance fertilization
Development, June 1, 2008; 135(11): 2001 - 2011.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
M. Shimada, Y. Yanai, T. Okazaki, Y. Yamashita, V. Sriraman, M. C. Wilson, and J. S. Richards
Synaptosomal-Associated Protein 25 Gene Expression Is Hormonally Regulated during Ovulation and Is Involved in Cytokine/Chemokine Exocytosis from Granulosa Cells
Mol. Endocrinol., October 1, 2007; 21(10): 2487 - 2502.
[Abstract] [Full Text] [PDF]

Home page
 J AndrolHome page
R. K. Naz and R. Sellamuthu
Receptors in Spermatozoa: Are They Real?
J Androl, September 1, 2006; 27(5): 627 - 636.
[Full Text] [PDF]

Home page
 J AndrolHome page
R. Januchowski, A. K. Breborowicz, H. Ofori, P. Jedrzejczak, L. Pawelczyk, and P. P. Jagodzinski
Detection of a Short CCR5 Messenger RNA Isoform in Human Spermatozoa
J Androl, September 1, 2004; 25(5): 757 - 760.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
Q. Zhang, K. Shimoya, K. Temma, T. Kimura, T. Tsujie, M. Shioji, K. Wasada, O. Fukui, S. Hayashi, T. Kanagawa, et al.
Expression of fractalkine in the Fallopian tube and of CX3CR1 in sperm
Hum. Reprod., February 1, 2004; 19(2): 409 - 414.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.