Human Reproduction, Vol. 17, No. 7, 1773-1776,
July 2002
© 2002 European Society of Human Reproduction and Embryology
Effects of hepatitis C virus on the apoptosis percentage of granulosa cells in vivo in women undergoing IVF: preliminary results
1 Service d'Histologie, 2 Unité Inserm U327, 3 Service de Virologie, 4 Service de Gynécologie–Obstétrique, CHU Bichat-Claude-Bernard, and 5 Service de Gynécologie–Obstétrique, Hôpital Rothschild, Paris, France
BACKGROUND: The aim of this study was to investigate the relationship between the apoptosis percentage of human luteinized granulosa cells (GC) and the presence of hepatitis C virus (HCV) in follicular fluid (FF). METHODS: GC were isolated from FF of 12 women undergoing 12 IVF cycles: six were HCV+ with active viral replication and six HCV– serving as controls. No male partner was HCV+. HCV detection and quantification were assessed by reverse transcriptase–polymerase chain reaction in serum, FF and embryo-incubation medium. GC were analysed by flow cytometry after propidium iodide staining to measure the percentages of apoptotic GC. Routine IVF parameters were tabulated. RESULTS: Mean ± standard deviation (SD) serum and FF HCV viral loads were 3.58 ± 4.25x106 and 0.14 ± 0.10x106 IU/ml respectively. Mean percentages of apoptotic GC from HCV+ and HCV– women were 3.08 ± 1.14 and 3.14 ± 1.40% respectively. No statistically significant difference was found between these two groups concerning GC apoptosis and when we compared all IVF parameters. No HCV RNA was detected in embryo incubation media after 2 days of culture. CONCLUSIONS: Comparing GC apoptosis percentages and usual IVF parameters in the HCV+ group versus the HCV– group, our preliminary study shows that active chronic HCV infection does not affect follicle development and IVF outcome in HCV+ women undergoing IVF. Furthermore, the risk of newborns becoming HCV-infected might not be increased by assisted reproductive technologies when performed in couples in which women are HCV+ and men HCV–.
Key words: apoptosis/hepatitis C virus/human granulosa cell/IVF
6 To whom correspondence should be addressed at: Service d'Histologie, Embryologie et Cytogénétique, CHU Bichat-Claude-Bernard, Université Paris 7 Denis-Diderot, 46, rue Henri-Huchard, 75877 Paris Cedex 18, France. E-mail: histo.survgen{at}bch.ap-hop-paris.fr
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