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Human Reproduction, Vol. 17, No. 8, 2054-2061, August 2002
© 2002 European Society of Human Reproduction and Embryology

Efficacy of plant-produced recombinant antibodies against HCG

S. Kathuria1,2,*, R. Sriraman1,2,*, R. Nath1,2, M. Sack1, R. Pal3, O. Artsaenko1,5, G.P. Talwar2, R. Fischer4 and R. Finnern4,6

1 Department of Molecular Biotechnology, 52074 Aachen, Germany, 2 Talwar Research Foundation, New Delhi 110068, 3 Immunoendocrinology Laboratory, National Institute of Immunology, New Delhi 110067, India and 4 Fraunhofer IME, Department of Molecular Biology, 52074 Aachen, Germany

BACKGROUND: Antibody engineering facilitates the construction of different antibody formats [single chain variable fragment (scFv), diabody, full-size chimeric monoclonal antibody] with ease. METHODS: We constructed recombinant antibodies against HCG, which is widely used in pregnancy testing and is also produced by a number of cancers. RESULTS: The recombinant antibodies were transiently expressed in tobacco leaves to levels of up to 40 mg of pure protein per kg fresh leaf weight. Enzyme linked immunosorbent assay (ELISA) and electrophoretic mobility assay (EMSA) confirmed antibody specificity for the beta subunit of ß-HCG. The efficacy was confirmed by inhibiting HCG induced testosterone production by Leydig cells in vitro and by blocking the HCG induced increase in mouse uterine weight in vivo. CONCLUSIONS: Passive immunization with recombinant HCG-specific antibodies may have clinical utility as (i) diagnostic and therapeutic tools for HCG-expressing cancers and (ii) contraceptive measures.

Key words: antibody engineering/HCG/plant expression system/recombinant antibodies

* These authors contributed equally to this work

5 Present address: Experimental Hepatology, University Düsseldorf, 40225 Düsseldorf, Germany

6 To whom correspondence should be addressed at: Pharmaceutical Product Development, Fraunhofer IME, Worringerweg 1,52074 Aachen, Germany. E-mail: finnern{at}molbiotech.rwth-aachen.de


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