Human Reproduction, Vol. 18, No. 12, 2654-2659,
December 2003
© 2003 European Society of Human Reproduction and Embryology
Survival of primordial follicles following prolonged transportation of ovarian tissue prior to cryopreservation
1 Laboratory of Reproductive Biology, Section 5712, 2 The Fertility Clinic, Section 4071, University Hospital of Copenhagen, Rigshospitalet, DK-2100 Copenhagen and 3 The Fertility Clinic, University Hospital of Aarhus, Skejby Sygehus, DK-8200 Aarhus, Denmark
4 To whom correspondence should be addressed at: Laboratory of Reproductive Biology, Section 5712, The Juliane Marie Center for Children, Women and Reproduction, Rigshospitalet, Blegdamsvej 9, DK-2100 Copenhagen, Denmark. e-mail: yding{at}rh.dk
BACKGROUND: Cryopreservation of ovarian tissue for fertility preservation is becoming increasingly common. Treatment of diseases that may deprive the ovaries of follicles is often performed at local hospitals that are without the necessary facilities and expertise to cryopreserve ovarian tissue. The aim of the present study was to evaluate whether primordial follicles of ovarian cortex survive transport for up to 4 h prior to cryopreservation. METHODS: Immediately after recovery of one ovary from each of four patients, the cortex was roughly isolated, placed in IVF culture medium, kept on ice and transported for 3–4 h to the centre where final dissection and cryopreservation took place. Transplantation of pieces of thawed ovarian cortex under the skin of ovariectomized immunodeficient mice for a period of 4 weeks was used to assess the survival of primordial follicles. RESULTS: After transplantation, ovarian tissue from each of the four patients contained surviving follicles. CONCLUSIONS: Transport of roughly isolated ovarian cortex cooled on ice for a period of up to 4 h allows survival of primordial follicles following cryopreservation and transplantation to immunodeficient mice.
Key words: cryopreservation/ovary/survival of follicles/transportation/xenotransplantation
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  I. Demeestere, P. Simon, S. Emiliani, A. Delbaere, and Y. Englert Orthotopic and heterotopic ovarian tissue transplantation Hum. Reprod. Update, November 1, 2009; 15(6): 649 - 665. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Keros, S. Xella, K. Hultenby, K. Pettersson, M. Sheikhi, A. Volpe, J. Hreinsson, and O. Hovatta Vitrification versus controlled-rate freezing in cryopreservation of human ovarian tissue Hum. Reprod., July 1, 2009; 24(7): 1670 - 1683. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H M Picton, S E Harris, W Muruvi, and E L Chambers The in vitro growth and maturation of follicles Reproduction, December 1, 2008; 136(6): 703 - 715. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Y. Andersen, M. Rosendahl, A. G. Byskov, A. Loft, C. Ottosen, M. Dueholm, K. L.T. Schmidt, A. N. Andersen, and E. Ernst Two successful pregnancies following autotransplantation of frozen/thawed ovarian tissue Hum. Reprod., October 1, 2008; 23(10): 2266 - 2272. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Soleimani, J. Van der Elst, E. Heytens, R. Van den Broecke, J. Gerris, M. Dhont, C. Cuvelier, and P. De Sutter Back muscle as a promising site for ovarian tissue transplantation, an animal model Hum. Reprod., March 1, 2008; 23(3): 619 - 626. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-M. Dolmans, B. Martinez-Madrid, E. Gadisseux, Y. Guiot, W. Y. Yuan, A. Torre, A. Camboni, A. Van Langendonckt, and J. Donnez Short-term transplantation of isolated human ovarian follicles and cortical tissue into nude mice Reproduction, August 1, 2007; 134(2): 253 - 262. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Jahnukainen, J. Ehmcke, S. D. Hergenrother, and S. Schlatt Effect of cold storage and cryopreservation of immature non-human primate testicular tissue on spermatogonial stem cell potential in xenografts Hum. Reprod., April 1, 2007; 22(4): 1060 - 1067. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F.J. Broekmans, J. Kwee, D.J. Hendriks, B.W. Mol, and C.B. Lambalk A systematic review of tests predicting ovarian reserve and IVF outcome Hum. Reprod. Update, November 1, 2006; 12(6): 685 - 718. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-M. Dolmans, N. Michaux, A. Camboni, B. Martinez-Madrid, A. Van Langendonckt, S. Annarita Nottola, and J. Donnez Evaluation of Liberase, a purified enzyme blend, for the isolation of human primordial and primary ovarian follicles Hum. Reprod., February 1, 2006; 21(2): 413 - 420. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Kvist, J. Thorup, A.G. Byskov, P.E. Hoyer, K. Mollgard, and C. Yding Andersen Cryopreservation of intact testicular tissue from boys with cryptorchidism Hum. Reprod., February 1, 2006; 21(2): 484 - 491. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Gook, D.H. Edgar, J. Borg, J. Archer, and J.C. McBain Diagnostic assessment of the developmental potential of human cryopreserved ovarian tissue from multiple patients using xenografting Hum. Reprod., January 1, 2005; 20(1): 72 - 78. [Abstract] [Full Text] [PDF]
|
|