Human Reproduction, Vol. 18, No. 12, 2660-2667,
December 2003
© 2003 European Society of Human Reproduction and Embryology
Restoration of fertility in infertile mice by transplantation of cryopreserved male germline stem cells
1 Horizontal Medical Research Organization, 3 Department of Pathology and Biology of Diseases, Graduate School of Medicine, Kyoto University and 2 The Institute of Physical and Chemical Research (RIKEN), Bioresource Center, Ibaraki, Japan
4 To whom correspondence should be addressed at: Horizontal Medical Research Organization, Graduate School of Medicine, Kyoto University 606-8501, Japan. e-mail: takashi{at}mfour.med.kyoto-u.ac.jp
BACKGROUND: The development of a spermatogonial transplantation technique has provided new possibilities for the treatment of male infertility. Previous studies have shown that spermatogonial stem cells could reinitiate spermatogenesis after cryopreservation and reintroduction into the seminiferous tubules of infertile recipient males, and this raised the possibility of banking frozen stem cells for male infertility treatment. It remains unknown, however, whether germ cells from freeze–thawed stem cells are fertile, leaving the possibility that the procedure compromises the integrity of the stem cells. METHODS AND RESULTS: Dissociated mouse testis cells were cryopreserved and transplanted into infertile recipient testes. The freeze–thawed testis cell populations contained higher concentrations of stem cells than fresh testis cell populations. Offspring were obtained from freeze–thawed stem cells transplanted into infertile males, and fertility restoration was more efficient in immature (5–10 days old) than in mature (6–12 weeks old) recipients. However, offspring were also obtained from infertile adult recipients using in-vitro microinsemination. CONCLUSIONS: This first successful application of frozen stem cell technology in the production of offspring by spermatogonial transplantation suggests the superiority of immature recipients for clinical applications. Thus, the combination of cryopreservation and transplantation of stem cells is a promising approach to overcome male infertility.
Key words: cryopreservation/infertility/stem cell/testis/transplantation
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  E. Goossens, M. De Rycke, P. Haentjens, and H. Tournaye DNA methylation patterns of spermatozoa and two generations of offspring obtained after murine spermatogonial stem cell transplantation Hum. Reprod., September 1, 2009; 24(9): 2255 - 2263. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. M Aponte, T. Soda, K. J Teerds, S C. Mizrak, H. J G van de Kant, and D. G de Rooij Propagation of bovine spermatogonial stem cells in vitro Reproduction, November 1, 2008; 136(5): 543 - 557. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Geens, E. Goossens, G. De Block, L. Ning, D. Van Saen, and H. Tournaye Autologous spermatogonial stem cell transplantation in man: current obstacles for a future clinical application Hum. Reprod. Update, March 1, 2008; 14(2): 121 - 130. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.P. Milazzo, L. Vaudreuil, B. Cauliez, E. Gruel, L. Masse, N. Mousset-Simeon, B. Mace, and N. Rives Comparison of conditions for cryopreservation of testicular tissue from immature mice Hum. Reprod., January 1, 2008; 23(1): 17 - 28. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Wyns, M. Curaba, B. Martinez-Madrid, A. Van Langendonckt, W. Francois-Xavier, and J. Donnez Spermatogonial survival after cryopreservation and short-term orthotopic immature human cryptorchid testicular tissue grafting to immunodeficient mice Hum. Reprod., June 1, 2007; 22(6): 1603 - 1611. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Jahnukainen, J. Ehmcke, S. D. Hergenrother, and S. Schlatt Effect of cold storage and cryopreservation of immature non-human primate testicular tissue on spermatogonial stem cell potential in xenografts Hum. Reprod., April 1, 2007; 22(4): 1060 - 1067. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Shinohara, M. Kato, M. Takehashi, J. Lee, S. Chuma, N. Nakatsuji, M. Kanatsu-Shinohara, and M. Hirabayashi Rats produced by interspecies spermatogonial transplantation in mice and in vitro microinsemination PNAS, September 12, 2006; 103(37): 13624 - 13628. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Goossens, V. Frederickx, G. de Block, A. van Steirteghem, and H. Tournaye Evaluation of in vivo conception after testicular stem cell transplantation in a mouse model shows altered post-implantation development Hum. Reprod., August 1, 2006; 21(8): 2057 - 2060. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Goossens, V. Frederickx, G. De Block, A. Van Steirteghem, and H. Tournaye Blastocyst development after assisted reproduction using spermatozoa obtained after testicular stem cell transplantation in mice Hum. Reprod., July 1, 2006; 21(7): 1759 - 1764. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Kanatsu-Shinohara, K. Inoue, J. Lee, H. Miki, N. Ogonuki, S. Toyokuni, A. Ogura, and T. Shinohara Anchorage-Independent Growth of Mouse Male Germline Stem Cells In Vitro Biol Reprod, March 1, 2006; 74(3): 522 - 529. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Kanatsu-Shinohara, N. Ogonuki, T. Iwano, J. Lee, Y. Kazuki, K. Inoue, H. Miki, M. Takehashi, S. Toyokuni, Y. Shinkai, et al. Genetic and epigenetic properties of mouse male germline stem cells during long-term culture Development, September 15, 2005; 132(18): 4155 - 4163. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kanatsu-Shinohara, H. Miki, K. Inoue, N. Ogonuki, S. Toyokuni, A. Ogura, and T. Shinohara Germline niche transplantation restores fertility in infertile mice Hum. Reprod., September 1, 2005; 20(9): 2376 - 2382. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Keros, B. Rosenlund, K. Hultenby, L. Aghajanova, L. Levkov, and O. Hovatta Optimizing cryopreservation of human testicular tissue: comparison of protocols with glycerol, propanediol and dimethylsulphoxide as cryoprotectants Hum. Reprod., June 1, 2005; 20(6): 1676 - 1687. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. E. Orwig and S. Schlatt Cryopreservation and Transplantation of Spermatogonia and Testicular Tissue for Preservation of Male Fertility J Natl Cancer Inst Monographs, March 1, 2005; 2005(34): 51 - 56. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Bahadur Ethics of testicular stem cell medicine Hum. Reprod., December 1, 2004; 19(12): 2702 - 2710. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Tournaye, E. Goossens, G. Verheyen, V. Frederickx, G. De Block, P. Devroey, and A. Van Steirteghem Preserving the reproductive potential of men and boys with cancer: current concepts and future prospects Hum. Reprod. Update, November 1, 2004; 10(6): 525 - 532. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kanatsu-Shinohara, S. Toyokuni, and T. Shinohara Transgenic Mice Produced by Retroviral Transduction of Male Germ Line Stem Cells In Vivo Biol Reprod, October 1, 2004; 71(4): 1202 - 1207. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Oatley, J. J. Reeves, and D. J. McLean Biological Activity of Cryopreserved Bovine Spermatogonial Stem Cells During In Vitro Culture Biol Reprod, September 1, 2004; 71(3): 942 - 947. [Abstract] [Full Text] [PDF]
|
|