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Human Reproduction, Vol. 18, No. 2, 257-261, February 2003
© 2003 European Society of Human Reproduction and Embryology

A simple, low cost and non-invasive method for screening Y-chromosome microdeletions in infertile men

I.E. Aknin-Seifer1,2, R.L. Touraine2, H. Lejeune3, J.L. Laurent1, B. Lauras2 and R. Levy1,3,4

1 Laboratoire de Biologie de la Reproduction, Hôpital Nord, 42055 Saint Etienne, 2 Laboratoire de Génétique Moléculaire, Faculté de médecine, porte123, 15 rue Ambroise Paré, 42023 Saint Etienne cedex 02 and 3 Département de Médecine de la Reproduction, Pavillon K1, place d’Arsonval, Hôpital Edouard Herriot, 69437 LYON, cedex 03, France

4 To whom correspondence should be addressed. e-mail: rachel.levy{at}chu-st-etienne.fr

BACKGROUND: Recent investigations emphasized a high prevalence of Y-chromosome microdeletions in men having severely impaired spermatogenesis. Screening of these men is recommended prior to assisted reproduction techniques. METHODS: The aim of this study was to define a reliable and efficient method to detect Y-chromosome deletions in infertile men. At first the feasibility of using a cytobrush to collect buccal cells as a source of DNA was tested. Then, a multiplex PCR in accordance with European recommendations (European Andrology Academia: EAA) was compared with a commercial kit. The test population consisted of 18 infertile male patients (with a known Y-deletion). Both buccal and blood cells were used for DNA extraction. A specific DNA extraction protocol was carried out on the buccal cells. RESULTS: Between 4–10 µg of DNA were retrieved per brush, allowing for several PCR attempts. The commercial kit failed to detect an AZFa deletion. Furthermore, markers sY130, sY133 and sY153, included in the kit, are not reliable. Both false negative and false positive results were generated by the commercial kit. CONCLUSION: A multiplex PCR performed pursuant to EAA recommendations is proposed. When the testing is conducted with DNA extracted from buccal cells, this protocol is simple, accurate and affordable.

Key words: male infertility/PCR/screening/Y chromosome


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I. E. Aknin-Seifer, H. Lejeune, R. L. Touraine, R. Levy, and Under the aegis of the SALF (Societe d'Andrologie
Y chromosome microdeletion screening in infertile men in France:a survey of French practice based on 88 IVF centres
Hum. Reprod., April 1, 2004; 19(4): 788 - 793.
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