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Human Reproduction, Vol. 18, No. 5, 1037-1046, May 2003
© 2003 European Society of Human Reproduction and Embryology

Accuracy of sperm–cervical mucus penetration tests in evaluating sperm motility in semen: a systematic quantitative review

Bolarinde Ola1,3,4, Masoud Afnan1, Spyros Papaioannou1, Khaldoun Sharif1, Lars Björndahl1 and Aravinthan Coomarasamy2

1 The Assisted Conception Unit and 2 Education Resource Centre, Birmingham Women’s Hospital, Birmingham B15 2TG and 3 Division of Reproductive and Child Health, The University of Birmingham, Birmingham B15 2TH, UK

4 To whom correspondence should be addressed at: Obstetrics and Gynaecology, The Jessop Wing, Sheffield Teaching Hospital NHS Trust, Tree Root Walk, Sheffield SI0 2SF, UK. e-mail: BolarindeOla{at}aol.com

BACKGROUND: Our objective was to determine the accuracy of in-vitro sperm penetration into cervical mucus or substitutes in evaluating sperm motility in semen. METHODS: This was a systematic quantitative review of test accuracy studies. The Cochrane library (2000:4), Medline (1966–2001), Embase (1988–2001) and SciSearch (1981–2001) were searched, in addition to manual searches of conference papers and bibliographies of known primary and review articles. Primary studies measuring in-vitro sperm penetration into cervical mucus, or substitutes (i.e. sperm–mucus penetration test, SMPT) and comparing results with sperm motility in semen were included. RESULTS: There were 18 primary diagnostic studies published in 17 papers, involving a total of 2580 samples. Fourteen primary diagnostic tests used vanguard distance as diagnostic criteria (SMPTvd) and the pooled likelihood ratio (LR) for positive (LR+) and negative (LR–) tests were 2.29 (1.82–2.87) and 0.52 (0.44–0.63) respectively. Four studies used diagnostic criteria based directly or indirectly on swim-up sperm count per high power field (SMPTsc) instead. Their pooled LR+ and LR– were 5.24 (3.36–8.18) and 0.15 (0.06–0.39) respectively. CONCLUSIONS: SMPTvd has a low accuracy in the evaluation of sperm motility in semen. However, SMPTsc was found to be more accurate. This method of using sperm concentration, instead of vanguard distance, as diagnostic criteria of in-vitro SMPT has potential as a useful laboratory-based sperm function test.

Key words: accuracy/hyaluronic acid/likelihood ratio/methyl cellulose/sperm–mucus penetration tests


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