Skip Navigation


Hum. Reprod. Advance Access originally published online on April 7, 2004
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF ) Freely available
Right arrow All Versions of this Article:
19/5/1222    most recent
deh218v1
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (13)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Kudo, Y.
Right arrow Articles by Ohama, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kudo, Y.
Right arrow Articles by Ohama, K.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Human Reproduction, Vol. 19, No. 5, 1222-1230, May 2004
© 2004 European Society of Human Reproduction and Embryology

Mechanisms regulating the expression of indoleamine 2,3-dioxygenase during decidualization of human endometrium

Yoshiki Kudo1,4, Tetsuaki Hara1, Takafumi Katsuki1, Aya Toyofuku1, Yuki Katsura1, Osamu Takikawa2, Tsuneo Fujii3 and Koso Ohama1

1 Department of Obstetrics and Gynecology, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, 2 Department of Pharmacology, Hokkaido University School of Medicine, Sapporo and 3 Department of Obstetrics and Gynecology, National Kure Medical Center, Hiroshima, Japan

4 To whom correspondence should be addressed at: Department of Obstetrics and Gynecology, Graduate School of Biomedical Sciences, Hiroshima University, Kasumi 1-2-3, Hiroshima 734-8551, Japan. e-mail: yoshkudo@hiroshima-u.ac.jp

BACKGROUND: Expression of the tryptophan catabolizing enzyme, indoleamine 2,3-dioxygenase, in the mouse placenta has been shown to be critical in preventing immunological rejection of the fetal allograft. To clarify the physiological importance of indoleamine 2,3-dioxygenase in human pregnancy, we have studied how the expression of this enzyme changes during decidualization of human endometrium at both the cell and tissue level. METHODS and RESULTS: The level of indoleamine 2,3-dioxygenase mRNA expression (determined by RT–PCR) was higher in decidual than in endometrial tissue. Uterine decidual tissue in ectopic pregnancy similarly showed increased mRNA expression. Immunohistochemistry demonstrated that indoleamine 2,3-dioxygenase protein immunoreactivity was found in glandular epithelium and in stromal cells. The intensity of this immunoreactivity was increased in decidualized tissue. In a cell culture model, the level of indoleamine 2,3-dioxygenase mRNA was suppressed specifically by progesterone-induced decidualization of isolated endometrial stromal cells. Indoleamine 2,3-dioxygenase protein abundance (determined by Western blot) was also decreased by progesterone-induced decidualization. However interferon-{gamma}, a potent stimulator of indoleamine 2,3-dioxygenase gene expression, increased the level of indoleamine 2,3-dioxygenase mRNA and protein in both non-decidualized and in decidualized cells. Indoleamine 2,3-dioxygenase activity (determined by measuring the concentration of tryptophan and its indoleamine 2,3-dioxygenase catabolite, kynurenine) was also decreased by progesterone-induced decidualization but enhanced following interferon-{gamma} treatment. Expression of other interferon-{gamma} inducible genes (STAT1 and tryptophanyl-tRNA synthetase) showed the same pattern as that of indoleamine 2,3-dioxygenase in tissue samples, but was not changed by decidualization in the cell culture model. CONCLUSIONS: These data suggest that despite suppression by progesterone, indoleamine 2,3-dioxygenase expression in endometrial stromal cells may increase during decidualization due to stimulation by interferon-{gamma} secreted by infiltrating leukocytes.

Key words: decidualization/endometrium/indoleamine 2,3-dioxygenase/interferon-{gamma}


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol Hum ReprodHome page
A. Germeyer, A. M. Sharkey, M. Prasadajudio, R. Sherwin, A. Moffett, K. Bieback, S. Clausmeyer, L. Masters, R. M. Popovici, A. P. Hess, et al.
Paracrine effects of uterine leucocytes on gene expression of human uterine stromal fibroblasts
Mol. Hum. Reprod., January 1, 2009; 15(1): 39 - 48.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
M. Takano, Z. Lu, T. Goto, L. Fusi, J. Higham, J. Francis, A. Withey, J. Hardt, B. Cloke, A. V. Stavropoulou, et al.
Transcriptional Cross Talk between the Forkhead Transcription Factor Forkhead Box O1A and the Progesterone Receptor Coordinates Cell Cycle Regulation and Differentiation in Human Endometrial Stromal Cells
Mol. Endocrinol., October 1, 2007; 21(10): 2334 - 2349.
[Abstract] [Full Text] [PDF]

Home page
 J Mol EndocrinolHome page
J J Brosens and B Gellersen
Death or survival - progesterone-dependent cell fate decisions in the human endometrial stroma.
J. Mol. Endocrinol., June 1, 2006; 36(3): 389 - 398.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
A. Toyofuku, T. Hara, T. Taguchi, Y. Katsura, K. Ohama, and Y. Kudo
Cyclic and characteristic expression of phosphorylated Akt in human endometrium and decidual cells in vivo and in vitro
Hum. Reprod., May 1, 2006; 21(5): 1122 - 1128.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
M. Hill, V. Pereira, C. Chauveau, R. Zagani, S. Remy, L. Tesson, D. Mazal, L. Ubillos, R. Brion, K. Ashgar, et al.
Heme oxygenase-1 inhibits rat and human breast cancer cell proliferation: mutual cross inhibition with indoleamine 2,3-dioxygenase
FASEB J, December 1, 2005; 19(14): 1957 - 1968.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
A. Honig, L. Rieger, J. Dietl, and U. Kammerer
Mechanisms regulating the expression of indoleamine 2,3-dioxygenase during decidualization of human endometrium
Hum. Reprod., November 1, 2004; 19(11): 2683 - 2684.
[Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.