Skip Navigation


Hum. Reprod. Advance Access originally published online on July 22, 2004
Human Reproduction 2004 19(9):2118-2125; doi:10.1093/humrep/deh367
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF ) Freely available
Right arrow All Versions of this Article:
19/9/2118    most recent
deh367v1
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (29)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Gutiérrez-Mateo, C.
Right arrow Articles by Navarro, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gutiérrez-Mateo, C.
Right arrow Articles by Navarro, J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Human Reproduction vol. 19 no. 9 © European Society of Human Reproduction and Embryology 2004; all rights reserved

Reliability of comparative genomic hybridization to detect chromosome abnormalities in first polar bodies and metaphase II oocytes

Cristina Gutiérrez-Mateo1,4, Dagan Wells2, Jordi Benet1, Jorge F. Sánchez-García1, Mercedes G. Bermúdez2, Itziar Belil3, Josep Egozcue1, Santiago Munné2 and Joaquima Navarro1

1 Departament de Biologia Cel.lular, Fisiologia i Immunologia, Unitat de Medicina, Universitat Autònoma de Barcelona, E-08193 Bellaterra, Spain, 2 The Institute for Reproductive Medicine and Science, St Barnabas Medical Center, 101 Old Short Hills Road, Suite 501, West Orange, NJ 07052, USA and 3 Servei de Medicina de la Reproducció, Institut Universitari Dexeus, Pg. Bonanova 89-91, E-08017, Barcelona, Spain

4 To whom correspondence should be addressed at: Departament de Biologia Cel.lular, Fisiologia i Immunologia, Unitat de Biologia, Facultat de Medicina, Universitat Autònoma de Barcelona, E-08193 Bellaterra, Spain. Tel: +34 935811175; Fax:+34 935811025; Email: cristina.gutierrez{at}uab.es; joaquima.navarro{at}uab.es

BACKGROUND: Preimplantation Genetic Diagnosis (PGD) using FISH to analyze up to nine chromosomes to discard chromosomally abnormal embryos has resulted in an increase of pregnancy rates in certain groups of patients. However, the number of chromosomes that can be analyzed is a clear limitation. We evaluate the reliability of using comparative genomic hybridization (CGH) to detect the whole set of chromosomes, as an alternative to PGD using FISH. METHODS and RESULTS: We have analysed by CGH both, first polar bodies (1PBs) and metaphase II (MII) oocytes from 30 oocytes donated by 24 women. The aneuploidy rate was 48%. Considering two maternal age groups, a higher number of chromosome abnormalities were detected in the older group of oocytes (23% versus 75%, P<0.02). About 33% of the 1PB-MII oocyte doublets diagnosed as aneuploid by CGH would have been misdiagnosed as normal if FISH with nine chromosome probes had been used. CONCLUSION: We demonstrate the reliability of 1PB analysis by CGH, to detect almost any chromosome abnormality in oocytes as well as unbalanced segregations of maternal translocations in a time frame compatible with regular in vitro fertilization (IVF). The selection of euploid oocytes could help to increase implantation and pregnancy rates of patients undergoing IVF treatment.

Key words: aneuploidy/comparative genomic hybridization/first polar body/oocyte/preimplantation genetic diagnosis


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Hum ReprodHome page
R. Garcia-Cruz, A. Casanovas, M. Brieno-Enriquez, P. Robles, I. Roig, A. Pujol, L. Cabero, M. Durban, and M. Garcia Caldes
Cytogenetic analyses of human oocytes provide new data on non-disjunction mechanisms and the origin of trisomy 16
Hum. Reprod., October 14, 2009; (2009) dep347v1.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
A. Obradors, E. Fernandez, M. Oliver-Bonet, M. Rius, A. de la Fuente, D. Wells, J. Benet, and J. Navarro
Birth of a healthy boy after a double factor PGD in a couple carrying a genetic disease and at risk for aneuploidy: Case Report
Hum. Reprod., August 1, 2008; 23(8): 1949 - 1956.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
E. Fragouli, D. Wells, A. Thornhill, P. Serhal, M.J.W. Faed, J.C. Harper, and J.D.A. Delhanty
Comparative genomic hybridization analysis of human oocytes and polar bodies
Hum. Reprod., September 1, 2006; 21(9): 2319 - 2328.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
C. Le Caignec, C. Spits, K. Sermon, M. De Rycke, B. Thienpont, S. Debrock, C. Staessen, Y. Moreau, J.-P. Fryns, A. Van Steirteghem, et al.
Single-cell chromosomal imbalances detection by array CGH.
Nucleic Acids Res., January 1, 2006; 34(9): e68 - e68.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
C. Gutierrez-Mateo, J. Benet, H. Starke, M. Oliver-Bonet, S. Munne, T. Liehr, and J. Navarro
Karyotyping of human oocytes by cenM-FISH, a new 24-colour centromere-specific technique
Hum. Reprod., December 1, 2005; 20(12): 3395 - 3401.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
C. Gutierrez-Mateo, L. Gadea, J. Benet, D. Wells, S. Munne, and J. Navarro
Aneuploidy 12 in a Robertsonian (13;14) carrier: Case report
Hum. Reprod., May 1, 2005; 20(5): 1256 - 1260.
[Abstract] [Full Text] [PDF]

Home page
 Hum Reprod UpdateHome page
F. Pellestor, T. Anahory, and S. Hamamah
The chromosomal analysis of human oocytes. An overview of established procedures
Hum. Reprod. Update, January 1, 2005; 11(1): 15 - 32.
[Abstract] [Full Text] [PDF]

Home page
 Hum Reprod UpdateHome page
L. Wilton
Preimplantation genetic diagnosis and chromosome analysis of blastomeres using comparative genomic hybridization
Hum. Reprod. Update, January 1, 2005; 11(1): 33 - 41.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
C. Gutierrez-Mateo, J. Benet, D. Wells, P. Colls, M.G. Bermudez, J.F. Sanchez-Garcia, J. Egozcue, J. Navarro, and S. Munne
Aneuploidy study of human oocytes first polar body comparative genomic hybridization and metaphase II fluorescence in situ hybridization analysis
Hum. Reprod., December 1, 2004; 19(12): 2859 - 2868.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.