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Hum. Reprod. Advance Access originally published online on February 10, 2005
Human Reproduction 2005 20(5):1364-1371; doi:10.1093/humrep/deh756
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© The Author 2005. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions{at}oupjournals.org

Mouse embryo development following IVF in media containing either L-glutamine or glycyl-L-glutamine

M.C. Summers1, L.K. McGinnis2, J.A. Lawitts3 and J.D. Biggers2,4

1 Reproductive Science Center, One Forbes Road, Lexington, MA, 02421 and Department of Obstetrics and Gynecology, University of Massachusetts Medical School, Worcester, MA, 10655, 2 Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA, 02115 and 3 Beth Israel Deaconess Hospital, Boston, MA 02115, USA

4 To whom correspondence should be addressed. Email: john_biggers{at}hms.harvard.edu

BACKGROUND: The development of the mouse zygote following fertilization in vitro in a KSOM-type medium containing either L-glutamine or glycyl-L-glutamine has been examined, and compared with the development of mouse zygotes produced by natural fertilization. METHODS: Mouse IVF, embryo culture and embryo transfer. RESULTS: Fertilization rates, development to the blastocyst stage, implantation rate, gross fetal development and fetal body weight are not different in a KSOM-type medium containing either L-glutamine or glycyl-L-glutamine. No evidence of abnormal fetal development, such as exencephaly, was observed. The replacement of L-glutamine with glycyl-L-glutamine favoured the development of relatively more inner cell mass cells than trophectoderm cells, and reduced the numbers of pyknotic and fragmented nuclei in the blastocysts that developed in vitro. CONCLUSIONS: There is no evidence that the presence of glutamine in the medium used for IVF influences significantly the subsequent development of the zygote. Replacing glutamine with glycyl-L-glutamine may be advantageous.

Key words: exencephaly/glutamine/glycylglutamine/IVF


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