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Hum. Reprod. Advance Access originally published online on April 28, 2005
Human Reproduction 2005 20(6):1619-1623; doi:10.1093/humrep/deh809
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© The Author 2005. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions{at}oupjournals.org

An improved mechanical technique for assisted hatching

Q.F. Lyu1,2,3, L.Q. Wu1,2, Y.P. Li2, Q. Pan1,2, D.E. Liu2, K. Xia1, D.S. Liang1, F. Cai1, Z.G. Long1, H.P. Dai1 and J.H. Xia1,2,4

1 National Laboratory of Medical Genetics of China and 2 Reproductive Medical Center of Xiangya Hospital, Central South University, Changsha, 410078, People's Republic of China

4 To whom correspondence should be addressed at: National Laboratory of Medical Genetics of China, Central South University, 110 Xiangya Road, Changsha, Hunan 410078, China. Email: nlmglcy{at}xysm.net

BACKGROUND: Varied clinical outcomes of assisted hatching (AH) have been reported. We attempt to investigate whether the size of the zona opening created by AH is adequate for blastocyst hatching, and, if not, set up a new method to improve it. METHODS: A new AH technique, long zona dissection (LZD), was established, and experiments were performed to compare the effects of different sizes of zona opening on complete hatching of blastocysts in mouse and human embryos in vitro. RESULTS: The LZD technique can create a long zona slit on early embryos, even blastocysts, with the slit size beyond two-thirds of zona diameter. Compared with three-dimensional partial zona dissection, LZD can significantly enhance the hatching speed and the rate of complete hatching of mouse blastocysts (93.9%). All (100%) human blastocysts completely hatched following LZD; however, when the slit size after AH was about two-fifths of zona diameter, more of the larger inner cell masses (ICM) became trapped by the zona opening during hatching than the smaller ICM (53.3 versus 12.5%, P=0.01). CONCLUSIONS: Zona opening of moderate size following AH is inadequate for the completion of blastocyst hatching in vitro; in some cases, however, it can be significantly improved by LZD.

Key words: assisted hatching/blastocyst/inner cell mass/safety/zona pellucida

3Present address: School of Life Science, Sun Yat-sen University, Guangzhou 510275, People's Republic of China


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