Skip Navigation


Hum. Reprod. Advance Access originally published online on March 24, 2005
Human Reproduction 2005 20(7):1975-1980; doi:10.1093/humrep/deh884
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF ) Freely available
Right arrow All Versions of this Article:
20/7/1975    most recent
deh884v1
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (50)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Chen, S.-U.
Right arrow Articles by Yang, Y.-S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chen, S.-U.
Right arrow Articles by Yang, Y.-S.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2005. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions{at}oupjournals.org

Observational clinical follow-up of oocyte cryopreservation using a slow-freezing method with 1,2-propanediol plus sucrose followed by ICSI

Shee-Uan Chen, Yih-Ron Lien, Hsin-Fu Chen, Li-Jung Chang, Yi-Yi Tsai and Yu-Shih Yang1

Department of Obstetrics and Gynecology, National Taiwan University Hospital and College of Medicine, Taipei, Taiwan

1 To whom correspondence should be addressed at: Department of Obstetrics and Gynecology, National Taiwan University Hospital, No. 7 Chung-Shan South Road, Taipei, Taiwan. ysyang{at}ha.mc.ntu.edu.tw

BACKGROUND: The value of oocyte cryopreservation remains controversial. Two major problems exist: poor survival and injury to the oocyte meiotic spindle after freezing and thawing. METHODS: For slow oocyte cryopreservation, we used 1.5 mol/l 1,2-propanediol and 0.3 mol/l sucrose. We waited 3 h after thawing for possible recovery of the meiotic spindles before performing ICSI. RESULTS: Forty-three women undergoing IVF or ICSI cycles cryopreserved some or all of their harvested oocytes; of these, 20 thawed their cryopreserved oocytes for personal use and one for donation. The survival rate of oocytes after thawing was 75%, with 67% of oocytes fertilizing normally after ICSI. All 21 cycles (100%) resulted in fertilization and embryo transfers. Seven pregnancies (33%) resulted. Four women delivered five babies with normal karyotypes. Three conceptions are ongoing. Compared to 38 cycles of frozen–thawed embryos at the pronuclear stage in the same period, the percentages of survival, pregnancy and implantation were similar. Additionally, four unmarried women with white blood cell diseases underwent oocyte freezing before preconditioning treatment for haematopoietic stem cell transplantation. CONCLUSIONS: This protocol achieved reproducible success of survival, fertilization and pregnancy for freezing and thawing of human oocytes. The 3 h post-thaw incubation could permit restoration of the meiotic spindles, thus facilitating normal fertilization.

Key words: cryopreservation/human oocytes/ICSI


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Hum ReprodHome page
U.-B. Wennerholm, V. Soderstrom-Anttila, C. Bergh, K. Aittomaki, J. Hazekamp, K.-G. Nygren, A. Selbing, and A. Loft
Children born after cryopreservation of embryos or oocytes: a systematic review of outcome data
Hum. Reprod., September 1, 2009; 24(9): 2158 - 2172.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
M. Xu, E. R. West-Farrell, R. L. Stouffer, L. D. Shea, T. K. Woodruff, and M. B. Zelinski
Encapsulated Three-Dimensional Culture Supports Development of Nonhuman Primate Secondary Follicles
Biol Reprod, September 1, 2009; 81(3): 587 - 594.
[Abstract] [Full Text] [PDF]

Home page
 Reproductive SciencesHome page
Shanshan Gao, Yuan Li, Xuan Gao, Jingmei Hu, Huijun Yang, and Z.-J. Chen
Spindle and Chromosome Changes of Human MII Oocytes During Incubation After Slow Freezing/Fast Thawing Procedures
Reproductive Sciences, April 1, 2009; 16(4): 391 - 396.
[Abstract] [PDF]

Home page
 Biol. Reprod.Home page
A. Eroglu, S. E. Bailey, M. Toner, and T. L. Toth
Successful Cryopreservation of Mouse Oocytes by Using Low Concentrations of Trehalose and Dimethylsulfoxide
Biol Reprod, January 1, 2009; 80(1): 70 - 78.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
L. Parmegiani, G.E. Cognigni, S. Bernardi, W. Ciampaglia, F. Infante, P. Pocognoli, C. T. de Fatis, E. Troilo, and M. Filicori
Freezing within 2 h from oocyte retrieval increases the efficiency of human oocyte cryopreservation when using a slow freezing/rapid thawing protocol with high sucrose concentration
Hum. Reprod., August 1, 2008; 23(8): 1771 - 1777.
[Abstract] [Full Text] [PDF]

Home page
 Hum Reprod UpdateHome page
D. A. Gook and D. H. Edgar
Human oocyte cryopreservation
Hum. Reprod. Update, November 1, 2007; 13(6): 591 - 605.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
L. De Santis, G. Coticchio, S. Paynter, D. Albertini, K. Hutt, I. Cino, M. Iaccarino, A. Gambardella, C. Flamigni, and A. Borini
Permeability of human oocytes to ethylene glycol and their survival and spindle configurations after slow cooling cryopreservation
Hum. Reprod., October 1, 2007; 22(10): 2776 - 2783.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
E. Van den Abbeel, U. Schneider, J. Liu, Y. Agca, J. K Critser, and A. Van Steirteghem
Osmotic responses and tolerance limits to changes in external osmolalities, and oolemma permeability characteristics, of human in vitro matured MII oocytes
Hum. Reprod., July 1, 2007; 22(7): 1959 - 1972.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
S.A. Nottola, G. Macchiarelli, G. Coticchio, S. Bianchi, S. Cecconi, L. De Santis, G. Scaravelli, C. Flamigni, and A. Borini
Ultrastructure of human mature oocytes after slow cooling cryopreservation using different sucrose concentrations
Hum. Reprod., April 1, 2007; 22(4): 1123 - 1133.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
S. Hashimoto, Y. Murata, M. Kikkawa, M. Sonoda, H. Oku, T. Murata, K. Sugihara, F. Nagata, Y. Nakaoka, A. Fukuda, et al.
Successful delivery after the transfer of twice-vitrified embryos derived from in vitro matured oocytes: A Case Report
Hum. Reprod., January 1, 2007; 22(1): 221 - 223.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
S.-U. Chen, C.-L. Chien, M.-Y. Wu, T.-H. Chen, S.-M. Lai, C.-W. Lin, and Y.-S. Yang
Novel direct cover vitrification for cryopreservation of ovarian tissues increases follicle viability and pregnancy capability in mice
Hum. Reprod., November 1, 2006; 21(11): 2794 - 2800.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
G. Coticchio, L. De Santis, G. Rossi, A. Borini, D. Albertini, G. Scaravelli, C. Alecci, V. Bianchi, S. Nottola, and S. Cecconi
Sucrose concentration influences the rate of human oocytes with normal spindle and chromosome configurations after slow-cooling cryopreservation*
Hum. Reprod., July 1, 2006; 21(7): 1771 - 1776.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
A. Borini, R. Sciajno, V. Bianchi, E. Sereni, C. Flamigni, and G. Coticchio
Clinical outcome of oocyte cryopreservation after slow cooling with a protocol utilizing a high sucrose concentration
Hum. Reprod., February 1, 2006; 21(2): 512 - 517.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
X.-H. Li, S.-U. Chen, X. Zhang, M. Tang, Y.-R. Kui, X. Wu, S. Wang, and Y.-L. Guo
Cryopreserved oocytes of infertile couples undergoing assisted reproductive technology could be an important source of oocyte donation: a clinical report of successful pregnancies
Hum. Reprod., December 1, 2005; 20(12): 3390 - 3394.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.