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Hum. Reprod. Advance Access originally published online on April 21, 2005
Human Reproduction 2005 20(8):2289-2294; doi:10.1093/humrep/dei032
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© The Author 2005. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions{at}oupjournals.org

Expression of inhibin {alpha}, glial cell line-derived neurotrophic factor and stem cell factor in Sertoli cell-only syndrome: relation to successful sperm retrieval by microdissection testicular sperm extraction

K. Fujita1, A. Tsujimura1,5, T. Takao1, Y. Miyagawa1, K. Matsumiya1, M. Koga2, M. Takeyama2, H. Fujioka3, K. Aozasa4 and A. Okuyama1

1 Department of Urology and 4 Department of Pathology, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita Osaka 565-0871, 2 Department of Urology, Osaka Central Hospital, Osaka, 530-0001 and 3 Department of Urology, Osaka Police Hospital, Osaka, 543-8502, Japan

5 To whom correspondence should be addressed. Email: akitsuji{at}uro.med.osaka-u.ac.jp

BACKGROUND: Microdissection testicular sperm extraction (TESE) has provided new hope for successful sperm retrieval to patients with Sertoli cell-only syndrome (SCO). We determined expression of the inhibin {alpha} subunit, glial cell line-derived neurotrophic factor (GDNF) and stem cell factor (SCF) in Sertoli cells obtained from patients with SCO immunohistochemically and compared expression rates with rates of microdissection TESE sperm retrieval. METHODS: Testicular biopsy specimens were obtained from 52 men with non-obstructive azoospermia who underwent microdissection TESE and were diagnosed with SCO by histological analysis. RESULTS: All specimens showed intense staining for the inhibin {alpha} subunit. Moderate or intense staining for GDNF was observed in 65.8% of specimens. All but one showed moderate or intense staining for SCF. Among specimens negative for GDNF, the sperm retrieval rate was significantly higher (100%) for specimens with intense staining for SCF than for specimens with no or moderate staining (30.7%) (P<0.05) for SCF. CONCLUSION: GDNF expression differs among patients with SCO. The sperm retrieval rate was high in cases of no staining for GDNF and intense staining for SCF.

Key words: GDNF/inhibin/microdissection TESE/SCF/Sertoli cell-only syndrome


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