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Hum. Reprod. Advance Access originally published online on September 1, 2006
Human Reproduction 2006 21(11):2978-2984; doi:10.1093/humrep/del253
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© The Author 2006. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

The CryoLoop facilitates re-vitrification of embryos at four successive stages of development without impairing embryo growth

Courtney B. Sheehan, Michelle Lane and David K. Gardner1

Research Department, Colorado Center for Reproductive Medicine, Englewood, CO, USA

1 To whom correspondence should be addressed at: Colorado Center for Reproductive Medicine, 799 East Hampden Avenue, Suite 520, Englewood, CO 80113, USA. E-mail: dgardner{at}colocrm.com

BACKGROUND: Vitrification has been shown to be an effective method of cryopreservation, but little is known about re-vitrification of embryos. This study investigated the effect of re-vitrification on mouse embryo preimplantation development and viability post-transfer. METHODS: Mouse embryos at the 1-cell stage were vitrified using the CryoLoop technique. Embryos were warmed and then re-vitrified successively at the 2-, 8-cell and blastocyst stages. The effects of multiple rounds of vitrification on development, differentiation and viability were assessed and compared with non-vitrified embryos. RESULTS: Development to the 8-cell stage on day 3 and blastocyst on day 5 were not affected by re-vitrification. However, better hatching rates were observed in the non-vitrified control group. Total cell number and the number of cells allocated to the inner cell mass (ICM) were not different between treatments. The percentage of ICM development was also not different between treatments. Implantation rate and fetal weights were the same between treatments. However, overall there were fewer fetuses per embryo transferred in the re-vitrified group. CONCLUSION: Re-vitrification of mouse embryos has minimal effect on preimplantation embryo development or implantation potential.

Key words: blastocyst/cryopreservation/embryo culture/vitrification


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B. Balaban, B. Urman, B. Ata, A. Isiklar, M.G. Larman, R. Hamilton, and D.K. Gardner
A randomized controlled study of human Day 3 embryo cryopreservation by slow freezing or vitrification: vitrification is associated with higher survival, metabolism and blastocyst formation
Hum. Reprod., September 1, 2008; 23(9): 1976 - 1982.
[Abstract] [Full Text] [PDF]


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