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Hum. Reprod. Advance Access originally published online on October 27, 2005
Human Reproduction 2006 21(3):605-609; doi:10.1093/humrep/dei375
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© The Author 2005. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Tumour necrosis factor-{alpha}, interleukin-6 and interleukin-8 do not promote adhesion of human endometrial epithelial cells to mesothelial cells in a quantitative in vitro model*

Sophie Debrock1,4, Bart De Strooper2, Sarah Vander Perre1, Joseph A. Hill3 and Thomas M D’Hooghe1

1 Leuven University Fertility Center, UZ Gasthuisberg, KU Leuven, Belgium, 2 Center for Human Genetics KU Leuven, Belgium and 3 The Fertility Centers of New England, Reading, MA, USA.

4 To whom correspondence should be addressed: Leuven University Fertility Center, UZ Gasthuisberg, Herestraat 49, B-3000 Leuven, Belgium E-mail: sophie.debrock{at}uz.kuleuven.ac.be

BACKGROUND: A key factor in the pathogenesis of endometriosis is the endometrial–peritoneal adhesion. To study the pathogenesis of endometriosis, a quantitative in vitro assay (QIVA) was developed to measure in vitro adhesion between human endometrial epithelial cells and mesothelial cells using commercially available cell lines. Using the QIVA, the hypothesis was tested that tumour necrosis factor-{alpha} (TNF-{alpha}), interleukin-6 (IL-6) and interleukin-8 (IL-8) promote adhesion of endometrial epithelial cells to mesothelial cells. METHODS: Mesothelial cells were pre-treated with TNF-{alpha}, IL-6 or IL-8 in various concentrations (ranging from 0 to 1000 IU/ml) for 24 h. Confluent endometrial epithelial cells were labelled with [35S]methionine, added to the confluent mesothelial cells and incubated for 1 h. After incubation, non-adhering cells were removed and adherent cells were solubilized and their [35S]methionine radioactivity was counted to quantify the adherence of endometrial epithelial cells to mesothelial cells. RESULTS: The in vitro adhesion of human endometrial epithelial cells to human mesothelial cells was inhibited in a dose-dependent manner by TNF-{alpha} (P = 0.0007), IL-6 (P < 0.0001) and IL-8 (P = 0.0004). CONCLUSIONS: Using a quantitative in vitro adhesion assay, we were unable to confirm our hypothesis that TNF-{alpha}, IL-6 and IL-8 promote the in vitro adhesion between endometrial epithelial cells and mesothelial cells.

Key words: cytokines/endometriosis/endometrium/in vitro adhesion assay/mesothelium

* In part presented at the VII World Congress on Endometriosis, San Diego, California, February 24–27, 2002 and at the 58th Annual Meeting of the ASRM Conference, Seattle, Washington, October 12–17, 2002.


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