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Letters to the editor |
One-step versus two-step culture of mouse preimplantation embryos
1 Colorado Center for Reproductive Medicine, Englewood, CO, USA and 2 Obstetrics and Gynaecology Department, The Queen Elizabeth Hospital, University of Adelaide, Adelaide, South Australia, Australia
3 To whom correspondence should be addressed at: E-mail: dgardner@colocrm.com
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Sir,
We would like to offer a different perspective on the study reported by Biggers et al. (2005)
whose findings are contradictory to our own previous studies on these embryo culture media systems.
In our previous publication comparing KSOMAA to sequential media (Gardner and Lane, 2002, 2003; Reed et al., 2003), we were careful to ensure that all conditions used were exactly the same for the medium being tested, e.g. the same volume of medium. This was done because in our extensive experience, the volume of medium has a significant impact on culture outcome if there are problems with the oil source used. Specifically, when suboptimal oil is used, embryo development increases with increasing volumes of medium. In the