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Human Reproduction 2006 21(7):1936-1939; doi:10.1093/humrep/del071
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© The Author 2006. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Letters to the editor

Reply: One-step versus two-step culture of mouse preimplantation embryos

John D. Biggers 1 , 5 , Lynda McGinnis 2 and Michael C. Summers 3 , 4

1 Department of Cell Biology, Harvard Medical School, Boston, MA 2 Department of Molecular and Integrated Physiology, University of Kansas Medical School, Kansas City, KS 3 Reproductive Science Center, Lexington, MA and 4 Department of Obstetrics and Gynecology, University of Massachusetts Medical School, Worcester, MA, USA

5 To whom correspondence should be addressed at: Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA. E-mail: john_biggers@hms.harvard.edu

The first 150 words of the full text of this article appear below.

Sir,

David Gardner and Michele Lane, who have been strong advocates of two-step protocols for the culture of human preimplantation embryos, have written the accompanying letter in which they offer a ‘different perspective’ on our recent paper (Biggers et al., 2005Go). We feel that we must respond to this letter since their so-called ‘different perspective’ is an attempt to challenge the validity of our work by claims that are misleading and confusing. Also, there are several instances where we are seriously misquoted or misinterpreted. For example, Gardner and Lane, in the fourth paragraph of their letter, make the following statement:

So irrespective of the paper by Biggers et al. (2005)Go, his own previous work (Biggers et al., 2004aGo), and that of others, means that changing embryos to fresh medium after 48 h is absolute.

Nowhere does our work support changing media at 48 h. . . . [Full Text of this Article]

Challenges to our methodology

Challenges to our interpretations

EDTA toxicity
Glutamine breakdown
Back-to-nature principle
Implications for the culture of preimplantation human embryos


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