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Hum. Reprod. Advance Access originally published online on April 13, 2006
Human Reproduction 2006 21(8):2136-2140; doi:10.1093/humrep/del097
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© The Author 2006. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Laser-assisted hatching increases pregnancy and implantation rates in cryopreserved embryos that were allowed to cleave in vitro after thawing: a prospective randomized study

Basak Balaban, Bulent Urman1, Kayhan Yakin and Aycan Isiklar

Assisted Reproduction Unit, American Hospital of Istanbul, Istanbul, Turkey

1 To whom correspondence should be addressed at: American Hospital of Istanbul, Guzelbahce sok No. 20, 34365 Nisantasi, Istanbul, Turkey. E-mail: burman{at}superonline.com

BACKGROUND: Cryopreservation of embryos may lead to zona hardening that may compromise in vivo hatching and implantation following thawing and transfer. Assisted hatching (AH) has been advocated as a means of assisting the natural hatching process and enhancing implantation. METHODS: The aim of this study was to assess in a prospective randomized manner the effect of laser-assisted hatching (LAH) on implantation as well as clinical and multiple pregnancy rates (the primary outcome) after the transfer of frozen–thawed embryos. All embryos were thawed the day before transfer, and LAH was performed the next day on embryos that cleaved. Control group consisted of embryos that were transferred without AH. RESULTS: The performance of LAH significantly increased implantation (9.9 versus 20.1%, P < 0.01), clinical pregnancy (27.3 versus 40.9, P < 0.05) and multiple pregnancy rates (16 versus 40.3%, P < 0.07). In the LAH group, significantly more excess embryos that were left in culture hatched in vitro. CONCLUSIONS: LAH improves the outcome of frozen–thawed embryo transfer when performed before transfer on embryos that were allowed to cleave.

Key words: cryopreservation/frozen–thawed embryo transfer/implantation/laser assisted hatching/multiple pregnancy/pregnancy rate


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