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Hum. Reprod. Advance Access originally published online on September 11, 2006
Human Reproduction 2007 22(1):221-223; doi:10.1093/humrep/del354
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© The Author 2006. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Successful delivery after the transfer of twice-vitrified embryos derived from in vitro matured oocytes: A Case Report

S. Hashimoto1,3, Y. Murata1, M. Kikkawa1, M. Sonoda1, H. Oku1, T. Murata2, K. Sugihara2, F. Nagata2, Y. Nakaoka2, A. Fukuda2 and Y. Morimoto1

1 IVF NAMBA CLINIC, The Centre for Reproductive Medicine and Infertility and 2 IVF OSAKA CLINIC, Osaka, Japan

3 To whom correspondence should be addressed at: The Centre for Reproductive Medicine and Infertility, IVF NAMBA CLINIC, 1-17-28, Minamihorie, Nishi-ku, Osaka, 550-0015, Japan. E-mail: hashimoto{at}ivfnamba.com

We report here the first case of successful pregnancy and delivery after the blastocyst transfer of twice-vitrified embryos produced following in vitro maturation (IVM) and ICSI. The patient received 5000 IU hCG on day 12 of the treatment cycle, and oocyte retrieval was carried out 36 h after hCG injection. A total of 22 immature oocytes were obtained. Following incubation for 26 h in IVM medium, 15 oocytes (68.2%) reached metaphase II stage. In total, 13 oocytes (86.7%) were fertilized after ICSI with the husband’s sperm, and 11 embryos at the pronuclear stage and two cleaved embryos on day 2 were vitrified because of thin endometrial thickness. Eight cryopreserved embryos at the pronuclear stage were warmed and cultured until the day 3 stage. Three embryos were transferred, and three embryos were twice vitrified. Unfortunately, these transferred embryos did not implant. Three twice-vitrified embryos were rewarmed and cultured until the day 5 stage, and two embryos were transferred. The second transfer attempt of twice-vitrified embryos resulted in the full-term delivery of a healthy infant. This case report demonstrates that twice-vitrified embryos, developed using an IVM protocol, retain the developmental competence for full-term, healthy infants.

Key words: in vitro maturation/PCOS/repeated cryopreservation/vitrification


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