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Hum. Reprod. Advance Access originally published online on October 26, 2006
Human Reproduction 2007 22(1):63-74; doi:10.1093/humrep/del356
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© The Author 2006. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Expression of ADAMTS-5/implantin in human decidual stromal cells: regulatory effects of cytokines

H. Zhu, P.C.K. Leung and C.D. MacCalman1

Department of Obstetrics and Gynecology, University of British Columbia, Vancouver, BC, Canada

1 To whom correspondence should be addressed at: Department of Obstetrics and Gynecology, Child and Family Research Institute, University of British Columbia, Room I3091-950, West 28th Avenue, Vancouver, BC, Canada V5Z 4H4. E-mail: cdmaccalman{at}hotmail.com

BACKGROUND: The restricted expression of ADAMTS-5 (A Disintegrin And Metalloproteinase with ThromboSpondin repeats-5) to the maternal–fetal interface in mice has led to this novel metalloproteinase being assigned the trivial name ‘implantin’. METHODS: As a first step in determining whether ADAMTS-5 also contributes to the implantation process in humans, we have examined the spatiotemporal expression of this ADAMTS subtype in the endometrium during the menstrual cycle and pregnancy by immunohistochemical analysis. A quantitative competitive PCR (QC-PCR) strategy and western blotting were subsequently used to determine whether interleukin (IL)-1beta and transforming growth factor (TGF)-beta1, two cytokines involved in the formation of the maternal–fetal interface, were capable of regulating ADAMTS-5 messenger RNA (mRNA) and protein levels in primary cultures of stromal cells isolated from first trimester decidual tissues. RESULTS: ADAMTS-5 expression in the stroma of the human endometrium correlates with decidualization of this cellular compartment in vivo. IL-1beta was found to increase (P < 0.05) whereas TGF-beta1 decreased (P < 0.05) ADAMTS-5 mRNA and protein levels in decidual stromal cell cultures in a concentration- and time-dependent manner. These regulatory effects were attenuated by function-perturbing antibodies directed against either cytokine. CONCLUSIONS: ADAMTS-5 expression is restricted to decidualized stromal cells of the human endometrium in vivo and is subject to regulation by cytokines in vitro.

Key words: ADAMTS-5/cytokines/decidua/human/implantin


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