Hum. Reprod. Advance Access originally published online on November 24, 2006
Human Reproduction 2007 22(2):558-566; doi:10.1093/humrep/del404
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Investigation of respiration of individual bovine embryos produced in vivo and in vitro and correlation with viability following transfer
1 Department of Genetics and Biotechnology, Danish Institute of Agricultural Sciences, Tjele 2 Department of Large Animal Sciences, The Royal Veterinary and Agricultural University, Frederiksberg C 3 Trans-Embryo Genetics, Brædstrup and 4 Unisense FertiliTech A/S, Aarhus C, Denmark
5 To whom correspondence should be addressed at: Research Centre for Reproductive Health, Department of Obstetrics and Gynaecology, Faculty of Health Sciences, The University of Adelaide, 2nd Floor Medical School South, Frome Road, SA 5005 Adelaide, Australia. E-mail: ana.sousalopes{at}adelaide.edu.au
BACKGROUND: Quantification of oxygen consumption by individual preimplantation embryos has the potential to improve embryo selection. This study investigated whether respiration rates of individual embryos are useful indicators of embryo viability. The effect of the Nanorespirometer on embryo viability was also evaluated. METHODS: The respiration rates of individual day 7 bovine in vivo- (n = 44) and in vitro-produced (n = 156) embryos were measured using the Nanorespirometer. In vivo-produced embryos were individually transferred to recipients. RESULTS: The respiration rates of in vivo-produced embryos increased with increasing morphological quality and stage of development (P < 0.05). Pregnancy rates on days 35 and 60 were 65 and 60%, respectively. The mean respiration rate did not differ significantly between embryos producing and not producing a pregnancy, but the transfer of embryos with respiration rates <0.78 nl/h, between 0.78 and 1.10 nl/h, and >1.10 nl/h resulted in 48, 100 and 25% pregnancy rate, respectively. The mean respiration rate of in vitro-produced embryos was higher than that of in vivo-produced embryos because of differences in the morphological quality and stage of development. CONCLUSION: The Nanorespirometer does not adversely influence embryo viability, but the sample size was too small to confirm the significance of the correlation observed between respiration rates and viability.
Key words: bovine embryo/embryo quality/Nanorespirometer/oxygen/respiration
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