Hum. Reprod. Advance Access originally published online on March 19, 2007
Human Reproduction 2007 22(6):1778-1788; doi:10.1093/humrep/dem032
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Assessment of the proliferative status of epithelial cell types in the endometrium of young and menopausal transition women
1 Department of Developmental and Molecular Biology 2 Department of Obstetrics and Gynecology and Women's Health 3 Department of Epidemiology and Population Health 4 Center for the Study of Reproductive Biology and Women's Health, Albert Einstein College of Medicine (AECOM), Bronx, NY, USA
5 To whom Correspondence should be addressed at: Department of Obstetrics and Gynecology and Women's Health, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461. Tel.: +1 718 430 3152; Fax: +1 718 430 8586; E-mail: glicktoro{at}aol.com
BACKGROUND: We determined protein and mRNA expressions of markers of normal human endometrial proliferation and hypothesized that dysregulation of the endometrial response to estradiol (E2) and progesterone would be observed in the older menopausal transition (MT) women compared with mid-reproductive age (MRA) controls.
METHODS: Endometrial biopsies were prospectively obtained from MRA and MT non-randomized healthy volunteers during proliferative (± exogenous E2) and secretory (MRA only) menstrual cycle phases. mRNA and/or nuclear protein expressions of proliferative markers (MKI67, PCNA and MCM2), cell-cycle regulators (cyclins A1, E1 and D1 and cyclin dependant kinase Inhibitor B; CCNA1, CCNE1, CCND1 and CDKN1B) and sex-steroid receptors [estrogen receptor (ER) and progesterone receptor (PR)] were assessed in endometrial lumen, gland and stroma.
RESULTS: MRA women had significantly higher proliferative than secretory expression of MKI67, PCNA, MCM2, CCNA1, CCNE1, ESR1 and PGR in lumen and gland (minimal stromal changes), whereas CDKN1B protein expression was higher during the secretory phase. E2-treatment of MT women led to relatively less MKI67 glandular protein expression compared with MRA women; no other age-related differences were observed.
CONCLUSION: Although the MT does not appear to alter the proliferative cell phenotype of endometrial epithelium and stroma, the data suggest that prior to the MT, age is associated with a decrease in some proliferative markers and steroid receptor expression status within different endometrial cell types.
Key words: cell cycle/endometrium/laser-capture/menopausal transition/proliferation
6 A.L.N. and M.A. contributed equally to this research, J.W.P. and N.F.S., co-senior authors.
7 Current address: UCLA Department of Pathology, 650 Charles Young Drive Sth, Los Angeles, CA 90095, USA
8 Current address: University of Cincinnati College of Medicine, P.O. Box 670526, Cincinnati, OH 45267-0526, USA
Submitted on September 8, 2006; resubmitted on December 18, 2006; accepted on January 8, 2007.