Skip Navigation


Hum. Reprod. Advance Access originally published online on May 18, 2007
Human Reproduction 2007 22(7):1991-1995; doi:10.1093/humrep/dem124
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF ) Freely available
Right arrow All Versions of this Article:
22/7/1991    most recent
dem124v1
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (2)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Hyun, C.-S.
Right arrow Articles by Lim, J.-H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hyun, C.-S.
Right arrow Articles by Lim, J.-H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2007. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Optimal ICSI timing after the first polar body extrusion in in vitro matured human oocytes

Chang-Seop Hyun, Jung-Ho Cha, Weon-Young Son1, San-Hyun Yoon, Kyung-Ae Kim and Jin-Ho Lim

Maria Infertility Hospital, Seoul, Republic of Korea

1 Correspondence address. McGill Reproductive Center, Department of Obstetrics and Gynecology, Royal Victoria Hospital, McGill University, Montreal, Quebec, Canada H3A 1A1. Tel: +514-843-1650; Fax: +514-843-1496; E-mail: weon-young.son{at}muhc.mcgill.ca

BACKGROUND: This study was conducted to investigate the fertilization and embryo development of human oocytes injected at different time intervals after extrusion of the first polar body (PB) following in vitro maturation (IVM) in IVM cycles. Also, we evaluated whether spindle imaging could serve as a tool to determine the optimal ICSI time.

METHODS: Oocytes were collected from 43 women with polycystic ovary syndrome. Metaphase I (MI) oocytes after in vitro culture for 24 h from germinal vesicle stage were subjected to ICSI according to time after first PB extrusion. The intervals were: within 1 h (n = 38); 1–2 h (n = 30); 2–4 h (n = 26);4–6 (n = 28) and 6–8 h (n = 40). In some MI oocytes, viable spindle location was evaluated using Polscope microscopy at different time intervals after first PB extrusion.

RESULTS: Fertilization rate of the MI oocytes injected within 1 h after first PB extrusion was low (15.8; 6/38) (P < 0.01 versus all other times). In contrast, the fertilization rate was 80, 92.3, 82.1 and 85% for oocytes injected 1–2, 2–4, 4–6 and 6–8 h after first PB extrusion, respectively. Development of good-quality embryos was not significantly different among all the groups. Interestingly, all the oocytes injected within 1 h after first PB extrusion were in Telophase I.

CONCLUSIONS: Human oocytes matured in vitro needed at least 1 h after first PB extrusion to complete nuclear maturation. Use of a live spindle imaging system can help to decide the timing of ICSI for oocytes matured in vitro.

Key words: in vitro maturation/ICSI timing/first polar body/spindle imaging

Submitted on November 17, 2006; resubmitted on April 1, 2007; accepted on April 13, 2007.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Hum ReprodHome page
W.-Y. Son, J.-T. Chung, R.-C. Chian, B. Herrero, E. Demirtas, S. Elizur, Y. Gidoni, C. Sylvestre, N. Dean, and S. L. Tan
A 38 h interval between hCG priming and oocyte retrieval increases in vivo and in vitro oocyte maturation rate in programmed IVM cycles
Hum. Reprod., September 1, 2008; 23(9): 2010 - 2016.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.