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Hum. Reprod. Advance Access originally published online on February 15, 2008
Human Reproduction 2008 23(4):783-791; doi:10.1093/humrep/den024
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© The Author 2008. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Identification of proteomic differences in asthenozoospermic sperm samples

Juan Martínez-Heredia1,2,3, Sara de Mateo1,2,3, José M. Vidal-Taboada1,2,3, José Luis Ballescà4 and Rafael Oliva1,2,3,5

1 Human Genetics Research Group, Genetics Unit, Faculty of Medicine, University of Barcelona, Casanova 143, 08036 Barcelona, Spain 2 IDIBAPS, Hospital Clínic i Provincial, Villarroel 170, 08036 Barcelona, Spain 3 Biochemistry and Genetics Service, Hospital Clínic i Provincial, Villarroel 170, 08036 Barcelona, Spain 4 Institut Clínic de Ginecologia, Obstetricia i Neonatología, Hospital Clínic i Provincial, Villarroel 170, 08036 Barcelona, Spain

5Correspondence address. E-mail: roliva{at}ub.edu

BACKGROUND: Asthenozoospermia is one of the most common findings present in infertile males, but its aetiology remains unknown in most cases. Present proteomic tools now offer the opportunity to identify proteins which are differentially expressed in asthenozoospermic semen samples and potentially involved in infertility.

METHODS: We compared the expression of 101 sperm protein spots in 20 asthenozoospermic samples to that of 10 semen donor controls using two-dimensional proteomic analysis.

RESULTS: Seventeen protein spots have been identified at different amounts in the asthenozoospermic samples compared with controls. These are cytoskeletal actin-B, annexin-A5, cytochrome C oxidase-6B, histone H2A, prolactin-inducible protein and precursor, calcium binding protein-S100A9 (2 spots), clusterin precursor, dihydrolipoamide dehydrogenase precursor, fumarate hydratase precursor, heat shock protein-HSPA2, inositol-1 monophosphatase, 3-mercapto-pyruvate sulfurtransferase/dienoyl-CoA isomerase precursor, proteasome subunit-PSMB3, semenogelin 1 precursor and testis expressed sequence 12. The detected amount of these proteins enabled the grouping of asthenozoospermic sperm samples in an unsupervised clustering analysis.

CONCLUSIONS: We have identified several proteins present at different amount in asthenozoospermic sperm samples. These proteins could be candidates towards the development of diagnostic markers, and open up the opportunity to gain further insight into the pathogenic mechanisms involved in asthenozoospermia.

Key words: 2D-PAGE/human/proteome/spermatozoa/spermatozoon

Submitted on July 3, 2007; resubmitted on October 20, 2007; accepted on October 26, 2007.


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