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Hum. Reprod. Advance Access originally published online on February 28, 2008
Human Reproduction 2008 23(5):1118-1127; doi:10.1093/humrep/den048
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© The Author 2008. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Identification of differentially expressed markers in human follicular cells associated with competent oocytes

Melanie Hamel1, Isabelle Dufort1, Claude Robert1, Catherine Gravel1, Marie-Claude Leveille2, Arthur Leader2 and Marc-Andre Sirard1,3

1 Département des Sciences Animales, Centre de Recherche en Biologie de la Reproduction, Université Laval, Québec, Canada G1K 7P4 2 Ottawa Fertility Centre, Ottawa, Canada

3 Correspondence address. E-mail: marc-andre.sirard{at}crbr.ulaval.ca

BACKGROUND: The development of an accurate method for selection of high-quality embryos is essential to achieve high pregnancy rates with single embryo transfer in human IVF. The developmental competence of the oocyte is acquired during follicle maturation and strong communication also exists between the follicular cells (FCs) and the oocytes; thus oocyte developmental competence may be determined by markers expressed in the surrounding FCs.

METHODS: From consenting patients (n = 40), FCs were recovered on a per follicle basis by individual follicle puncture. Hybridization analyses using a custom-made complementary DNA microarray containing granulosa/cumulus expressed sequence tags (ESTs) from subtracted libraries and an Affymetrix GeneChip® were performed to identify specific genes expressed in follicles leading to a pregnancy. The selected candidate genes were validated by quantitative-PCR (Q-PCR).

RESULTS: Subtractive libraries prepared from pooled samples representing pregnant versus non-pregnant patients produced 1694 ESTs. Hybridization data analysis discriminated 115 genes associated with competent follicles. Selected candidates were confirmed by Q-PCR: 3-beta-hydroxysteroid dehydrogenase 1 (P = 0.0078), Ferredoxin 1 (P = 0.0203), Serine (or cysteine) proteinase inhibitor clade E member 2 (P = 0.0499), Cytochrome P450 aromatase (P = 0.0359) and Cell division cycle 42 (P = 0.0396).

CONCLUSIONS: Microarray technologies are useful to mine the transcriptome of FCs expressed in follicles associated with competent oocytes and could be used to improve embryo selection with the objective of successful single embryo transfer.

Key words: competent oocyte/follicle/granulosa cells/multiple pregnancies/gene expression

Submitted on August 6, 2007; resubmitted on January 7, 2008; accepted on January 26, 2008.


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