Hum. Reprod. Advance Access originally published online on March 7, 2008
Human Reproduction 2008 23(5):1193-1199; doi:10.1093/humrep/den069
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Do we need to search for gr/gr deletions in infertile men in a clinical setting?
1 Research Centre for Reproduction and Genetics, Vrije Universiteit Brussel, Laarbeeklaan 101, 1090 Brussels, Belgium 2 Centre for Medical Genetics, Universitair Ziekenhuis Brussel, Laarbeeklaan 101, 1090 Brussels, Belgium 3 Centre for Reproductive Medicine, Universitair Ziekenhuis Brussel, Laarbeeklaan 101, 1090 Brussels, Belgium 4 Centre for Outcomes Research and Laboratory for Experimental Surgery, Universitair Ziekenhuis Brussel, Vrije Universiteit Brussel, Laarbeeklaan 101, 1090 Brussels, Belgium
5 Correspondence address. Tel: +32-2-477-64-69; Fax: +32-2-477-68-60; E-mail: katrien.stouffs{at}uzbrussel.be
BACKGROUND: Partial deletions of the AZFc region of the Y chromosome such as gr/gr deletions have been detected in infertile patients as well as in control groups. The impact of these gr/gr deletions on the etiology of male infertility remains unknown. In the present study, we investigated the presence of gr/gr deletions in Caucasian men.
METHODS: gr/gr deletions were analyzed by using markers sY1291, sY1191 and sY1197 and by investigating the presence of single nucleotide variants (SNV) in DAZ and CDY1 genes in patients with azoospermia (n = 44), cryptozoospermia (n = 51) or severe oligozoospermia (n = 92). Control groups consisted of men with normal spermatogenesis on testicular biopsy (n = 33), normozoospermia (n = 278) or proven fertility (n = 83).
RESULTS: We observed 20 gr/gr deletions, with eight in infertile patients (4.3%) and 12 in the control groups (3.0%), which was not significantly different. DAZ SNV analysis revealed eight different deletion patterns in patients and controls.
CONCLUSIONS: In the present study, no significant differences in the frequency of gr/gr deletions between different patient and control groups were observed. We concluded that the relationship between gr/gr deletions and male infertility remains unclear and that it is too early to systematically test for gr/gr deletions for infertile couples seeking assisted reproduction treatment.
Key words: gr/gr deletions/male infertility/microdeletion/Y chromosome
Submitted on September 5, 2007; resubmitted on February 4, 2008; accepted on February 12, 2008.
CiteULike 
Connotea 
Del.icio.us What's this?
Related articles in Hum. Reprod.:
- Editor's Choice
Hum. Reprod. 2008 23: 1003.[Extract] [Full Text]