Skip Navigation


Hum. Reprod. Advance Access originally published online on June 20, 2009
Human Reproduction 2009 24(10):2649-2655; doi:10.1093/humrep/dep224
This Article
Right arrow Full Text
Right arrow Full Text (PDF )
Right arrow Supplementary Data
Right arrow All Versions of this Article:
24/10/2649    most recent
dep224v1
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Jaroudi, S.
Right arrow Articles by SenGupta, S. B.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jaroudi, S.
Right arrow Articles by SenGupta, S. B.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2009. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Expression profiling of DNA repair genes in human oocytes and blastocysts using microarrays

Souraya Jaroudi1, Georgia Kakourou1, Suzanne Cawood2, Alpesh Doshi2, Domenico M. Ranieri2, Paul Serhal2, Joyce C. Harper1 and Sioban B. SenGupta1,3

1 UCL Centre for PGD, Institute for Women's Health, University College London, 86-96 Chenies Mews, London WC1E 6HX, UK 2 The Assisted Conception Unit, University College Hospital, The New Wing Eastman Dental Hospital, 256 Gray's Inn Road, London WC1X 8LD, UK

3 Correspondence address. E-mail: s.sengupta{at}ucl.ac.uk

BACKGROUND: The early preimplantation embryo relies on mRNA and protein from the oocyte to detect DNA damage and activate DNA repair, cell cycle arrest or apoptosis. Expression of some repair genes has been detected in mammalian oocytes and embryos; however, little is known about DNA repair gene expression in human blastocysts. In this study, DNA repair gene expression was investigated in human oocytes and blastocysts to identify the pathways involved at these stages and detect potential differences in repair mechanisms pre- and post-embryonic genome activation.

METHODS: Triplicate sets of pooled metaphase II oocytes or blastocysts were processed for analysis using the Human Genome Survey Microarrays V2.0 (Applied Biosystems).

RESULTS: Of 154 DNA repair genes investigated, 109 were detected in blastocysts and 107 in oocytes. Among differentially expressed DNA repair genes, 40/55 (73%) had lower expression levels in blastocysts compared with oocytes (P < 0.05, fold change >3).

CONCLUSION: Despite experimental limitations due to culture or freezing and thawing of samples, large numbers of repair genes were detected indicating that all DNA repair pathways are potentially functional in human oocytes and blastocysts. The higher mRNA level for most repair genes in oocytes compared with blastocysts ensures sufficient availability of template until embryonic genome activation.

Key words: DNA repair/gene expression/human blastocyst embryo/human oocyte/microarrays

Submitted on February 3, 2009; resubmitted on May 15, 2009; accepted on May 27, 2009.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.