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Hum. Reprod. Advance Access originally published online on December 5, 2008
Human Reproduction 2009 24(3):732-740; doi:10.1093/humrep/den423
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Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology 2008

The experience of two European preimplantation genetic diagnosis centres on human leukocyte antigen typing

Hilde Van de Velde1,5, Martine De Rycke2, Caroline De Man2, Kim De Hauwere2, Francesco Fiorentino3, Semra Kahraman3, Guido Pennings4, Willem Verpoest1, Paul Devroey1 and Inge Liebaers2

1 Centre for Reproductive Medicine, UZ Brussel, Laarbeeklaan 101, 1090 Brussels, Belgium 2 Centre for Medical Genetics, UZ Brussel, Laarbeeklaan 101, 1090 Brussels, Belgium 3 Centre for Preimplantation Genetic Diagnosis, "GENOMA"—Molecular Genetics Laboratories, Rome, Italy 4 Department of Philosophy and Moral Science, Bioethics Institute Ghent, Ghent University, Blandijnberg, 2, 9000 Ghent, Belgium

5 Correspondence address. Tel: +32-2-477-6696; Fax: +32-2-477-6692; E-mail: hilde.vandevelde{at}uzbrussel.be

BACKGROUND: Two European centres report on human leukocyte antigen (HLA) typing of preimplantation embryos for haematopoietic stem cell (HSC) transplantation: ‘UZ Brussel’ in Brussels and ‘Genoma’ in Rome. Both centres have 6 years’ experience with technical and clinical aspects of this type of genetic analysis on single blastomeres.

METHODS: Both centres apply a similar technique for preimplantation HLA typing using short tandem repeats linked to the HLA locus in multiplex PCR for haplotyping.

RESULTS: At present, a conclusive HLA diagnosis could be assured in 92.8% and 90.3% of the embryos at UZ Brussel and at Genoma, respectively. The implantation rates were 32.4% and 28.2%, respectively, and the birth rates per cycle were 9.4% and 18.6%, respectively. The HLA programme at UZ Brussel and at Genoma resulted in the birth of 9 babies and 3 successful HSC transplantations, and 42 babies and 7 successful HSC transplantations, respectively, so far.

CONCLUSIONS: Drastic embryo selection for preimplantation HLA typing (in theory 1/4 for HLA, 1/8 for HLA in combination with sexing for X-linked recessive diseases, 3/16 for HLA in combination with autosomal recessive disorders) resulted overall in the birth of 51 babies (15.9% live birth rate per started cycle) in two European centres.

Key words: preimplantation HLA typing/preimplantation genetic diagnosis/HLA matching/short tandem repeats/live birth rate

Submitted on April 25, 2008; resubmitted on October 30, 2008; accepted on November 4, 2008.


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