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Hum. Reprod. Advance Access originally published online on December 23, 2008
Human Reproduction 2009 24(4):929-935; doi:10.1093/humrep/den429
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Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology 2008

Rhesus macaque embryos derived from MI oocytes maturing after retrieval display high rates of chromosomal anomalies

Cathérine Dupont1,2,6, Barry D. Bavister2,4, D. Randall Armant2,3,5 and Carol A. Brenner1,2

1 Department of Physiology, School of Medicine, Wayne State University, Detroit, MI, USA 2 Department of Obstetrics and Gynecology, School of Medicine, Wayne State University, Detroit, MI, USA 3 Department of Anatomy and Cell Biology, School of Medicine, Wayne State University, Detroit, MI, USA 4 Caribbean Primate Research Center, Sabana Seca, Puerto Rico 5 Program in Reproductive and Adult Endocrinology, NICHD, NIH, DHHS, Bethesda, MD, USA

6 Correspondence address. E-mail: cdupont{at}med.wayne.edu

BACKGROUND: Rhesus macaque and human preimplantation embryos display similar rates of chromosomal abnormalities. The aim of this study was to determine whether embryos developing from MI oocytes that mature post-retrieval display more chromosomal anomalies than those embryos that are generated from oocytes that are at MII at the time of retrieval.

METHODS: Rhesus macaque oocytes were obtained after hormonal ovarian stimulation. Immediately after retrieval, the oocytes were classified according to their maturational status. Following in vitro fertilization, Day 3 embryos with good morphology and development derived from oocytes maturing post-retrieval and those from oocytes that were mature at the time of retrieval were cytogenetically assessed using a five-color fluorescent in situ fluorescent hybridization assay developed for rhesus macaque chromosomes homologous to human chromosomes 13, 16, 18, X and Y.

RESULTS: Blastomeres from 53 embryos were analyzed. Of the 27 embryos that developed from oocytes that were mature at collection, 18 embryos were chromosomally normal (66.7%), while from the 26 embryos that developed from oocytes that matured post-retrieval, only 9 embryos were chromosomally normal (34.6%).

CONCLUSIONS: These results indicate that embryos developing from oocytes maturing post-retrieval display high rates of chromosomal abnormalities and have therefore a reduced developmental competence. As a result, the clinical relevance of using immature oocytes that are retrieved after stimulated cycles in human IVF warrants further investigation.

Key words: aneuploidy/embryo/FISH/in vitro maturation/monkey

Submitted on August 4, 2008; resubmitted on October 24, 2008; accepted on November 5, 2008.


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