Human Reproduction, Vol. 5, No. 8, pp. 987-989, 1990
© 1990 European Society of Human Reproduction and Embryology
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Mini-Percoll: a new method of semen preparation for IVF in severe male factor infertility
Department of Obstetrics and Gynecology, University of California Irvine (UCI), Orange, CA 92668, USA
Correspondence: 1To whom correspondence should be addressed
Conventional methods of semen preparation for IVF, such as swim-up sedimentation, regular Percoll gradients, are not very effective in the treatment of severe oligoasthenozoo-spermia (total motile count <5 x 106). For these cases, a new method of sperm preparation consisting of a reduced volume of a discontinuous Percoll gradient (mini-Percoll) has been utilized. The results obtained were analysed by comparing mini-Percoll versus resuspension in two groups of patients with severe male factor infertility. In the mini-Percoll group, a statistically significant improvment (P < 0.001) was obtained in the post-treatment seminal parameters of motility, progression and proportion of normal forms. In the resuspension group (n = 12), only eight of 108 oocytes inseminated were fertilized (7%), while in the mini-Percoll group (n = 29), 124 out of 336 oocytes were fertilized (40%) (P < 0.001). Furthermore, in the mini-Percoll group, a higher pregnancy rate (25 versus 7%) and low abortion rate (22 versus 33%) were observed. Although more studies are necessary to confirm these preliminary data, mini-Percoll seems to be a valid laboratory tool for semen samples of extremely poor quality.
Key words: discontinuous Percoll gradient/male factor/oligo-asthenozoospermia/resuspension
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