Human Reproduction, Vol. 6, No. 7, pp. 1017-1021, 1991
© 1991 European Society of Human Reproduction and Embryology
review-article |
Premature chromosome condensation as a sign of oocyte immaturity*
Dept. Biologia Cel·lular i Fisiologia, Universitat Autònoma de Barcelona 08193 Bellaterra, Barcelona, Spain
Correspondence: 1To whom correspondence should be addressed
In this work we report the possibility that oocyte immaturity is associated with premature chromosome condensation (PCC) after in-vitro fertilization (IVF). Using a murine model, we have related PCC and endoreduplicated-like oocytes to oocyte immaturity as a basis for a prognosis in oocyte immaturity problems. The cytogenetic analysis was performed in 511 embryos obtained from immature oocytes that were directly fertilized in vitro and in 1363 embryos obtained from immature oocytes that were matured in vitro with different concentrations of human chorionic gonadotrophin (HCG) added to the culture medium. As a control we used 507 embryos obtained from freshly ovulated oocytes. PCC at the G1-phase-(G1-PCC) was observed only when immature oocytes were immediately fertilized in vitro (45.4%) and PCC at the S-phase (S-PCC) only when using in-vitro matured oocytes with the highest HCG concentration (3.3%). Neither G1-PCC nor S-PCC were found in the control group. Endoreduplicated-like oocytes appeared In a significant percentage (27.3%) only in the immature group. Immature oocytes yielded a low fertilization rate (16.6%) while in-vitro maturation seemed to confer a higher fertilization capacity compared to the control group (90.1% versus 78.2%). The possible correlation between PCC and oocyte immaturity provides new prospects in the determination of human IVF failures of unknown origin. Thus, when a problem of oocyte immaturity is diagnosed through the presence of PCC, a special programme of in vitro oocyte maturation, such as a longer preincubation time or addition of hormones to the media, would be recommended.
Key words: IVF/oocyte immaturity/premature chromosome condensation
*This work is based on a free communication presented at the II Joint Meeting ESCO-ESHRE, August 29th-September 1st 1990, Milan, Italy.
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