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Human Reproduction, Vol. 7, No. 5, pp. 692-700, 1992
© 1992 European Society of Human Reproduction and Embryology

other

The expression of retinoic acid receptors in cultured human endometrial stromal cells and effects of retinoic acid

Prentice Andrew1,4,5, C. Jane Matthews2, Eric J. Thomas3 and Christopher P.F. Redfern1,5

1Medical Molecular Biology Group, (Departments of Obstetrics & Gynaecology and Dermatology), University of Newcastle upon Tyne 2Department of Physiological Sciences, University of Newcastle upon Tyne 3Department of Human Reproduction and Obstetrics, University of Southampton

Correspondence: 5To whom correspondence should be addressed

Patterns of expression of retinoic acid receptors (RAR) in cultures of human endometrial stromal cells are described. Transcripts for all three classses of RAR were expressed in these cells but RAR-{beta} was expressed at a low level by comparison with RAR-{alpha} and RAR-{gamma}. The abundance of RAR-{beta}transcripts was elevated by treating the cells with retinoic acid, but there was no effect on the level of expression of RAR-{alpha} and RAR-{gamma}. The induction of RAR-{beta} by retinoic acid was detectable within 4 h and at low concentrations of retinoic acid (10-10 M). Adenosine 3': 5'-cyclic monophosphate (cAMP) analogues and forskolin, an adenylate cyclase activator, had no effect on the retinoic acid-mediated induction of RAR-{beta}, contrary to recent observations on embryonal carcinoma cells. However, the phosphodiesterase inhibitor, 3-isobutyl-l-methyI-xanthine (EBMX), forskolin and 8-bromo-cAMP depressed basal levels of RAR-{beta} expression. These data suggest that endometrial stromal cells may be a target tissue of retinoic acid in vivo, and imply a role for retinoic acid in the cyclical differentiation of human endometrium.

Key words: endometrium/inductiori/retinoic acid/retinoic acid receptors/stromal cells

4Present address: Department of Obstetrics and Gynaecology, University of Cambridge Clinical School, Rosie Maternity Hospital Robinson Way, Cambridge, CB2 2SW, UK


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