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Human Reproduction, Vol. 8, No. 1, pp. 127-129, 1993
© 1993 European Society of Human Reproduction and Embryology


case-report

CASE REPORT: A monozygotic twin pregnancy after application of zona rubbing on a frozen—thawed blastocyst

M. Nijs1, P. Vanderzwalmen, G. Segal-Bertin, L. Geerts, E. van Roosendaal, L. Segal, A. Schoysman-Deboeck and R. Schoysman

IVF Centre, Van Helmont Ziekenhuis Vaartstraat 42, B 1800 Vilvoorde, Belgium

Correspondence: 1To whom correspondence should be addressed

Assisted hatching techniques enhance the success rate of implantation of in-vitro fertilized human embryos. We report here the successful transfer of a frozen—thawed human blastocyst on which we applied a non-invasive zona rubbing technique (reduction of the thickness of the zona pellucida by gentle rubbing with a microneedle). The implantation in the uterus led to the delivery of healthy monozygotic twins.

Key words: assisted hatching/cryopreservation/human blastocyst/in-vitro fertilization/zona rubbing


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