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Human Reproduction, Vol. 8, No. 12, pp. 2180-2184, 1993
© 1993 European Society of Human Reproduction and Embryology

review-article

Fertilization and early embryology: Intracytoplasmic single sperm injection of 1-day-old unfertilized human oocytes

Z.P. Nagy1, H. Joris, J. Liu, C. Staessen, P. Devroey and A.C. van Steirteghem

Centre for Reproductive Medicine, University Hospital, Vrije Universiteit Brussel Laarbeeklaan 101, B-1090 Brussels, Belgium

Correspondence: 1To whom correspondence should be addressed

Although the average fertilization rate in most in-vitro fertilization (IVF) centres is ~60–70%, there are cases of complete or virtually complete fertilization failures. The aim of our work was to study the fertilization and the subsequent cleavage characteristics of 1-day-old human oocytes treated by intracytoplasmic single sperm injection (ICSI) after failing to fertilize during the standard IVF procedure. A total of 115 metaphase II 1-day-old unfertilized oocytes were collected from 23 patients. No additional treatment was applied to the oocytes or to the semen sample. A single spermatozoon from the patient's husband was injected into the cytoplasm of each of these oocytes 21–33 h after ovum retrieval. Injected oocytes were observed at 16–18 h and again 42–44 h after the ICSI procedure. Of the injected oocytes, 92% (n = 106) were intact after ICSI, 38% (n = 44) had two distinct pronuclei and there was no difference in the fertilization rate of oocytes when andrological and non-andrological patients were compared. Similarly, there was no difference in the fertilization rate after ICSI where patients with acceptable or good (> 15%) fertilization after standard IVF were compared to patients who had poor (<15%) fertilization after IVF. There was no significant difference in the sperm concentration or in the progressive forward motility (a + b motility) in these groups except where a + b motility of andrological and non-adrological patients was compared. The majority (84%) of the normally fertilized oocytes cleaved and most (77%) of these embryos showed <20% fragmentation 2 days after the ICSI procedure. From this study it can be concluded that 1-day-old metaphase II oocytes which have failed to be fertilized after standard IVF procedure can be fertilized and cleave when ICSI is performed on them the day after oocyte retrieval.

Key words: aged human oocyte/ICSI/micro-injection/reinsemination


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