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Human Reproduction, Vol. 9, No. 4, pp. 664-668, 1994
© 1994 European Society of Human Reproduction and Embryology

other

The effect of some proteinase inhibitors on liquefaction of human semen

Yoshifumi Matsuda1,3, Shigeru Oshio2, Tsunetada Yazaki2, Takashi Umeda2 and Sumiyuki Akihatna1

1Department of Biochemistry, Meiji College of Pharmacy Nozawa 1-35-23, Setagaya-ku, Tokyo 154, Japan 2Department of Urology, Teikyo University School of Medicine Kaga, Itabashi-ku, Tokyo, Japan

Correspondence: 3To whom correspondence should be addressed

This study examined the effect of some proteinase inhibitors on liquefaction of human semen. It revealed that a strong plasmin inhibitor, 6-amidino-2-naphtyl-6-guanidinobenzoate dihydrochloride (Fusan) showed a significant inhibition of liquefaction, while t-amino caproic acid (t-ACA) showed a weak retardation effect. In terms of sperm quality after liquefaction, Fusan (10 mM), ethyl diamine tetra-acetic acid (EDTA) and Urinastatin completely inhibited sperm motility. Fusan (1 mM) and Lima bean trypsin inhibitor (LBTI) decreased sperm motility significantly, while leupeptin and t-ACA had little effect. Leupeptin, LBTI, t-ACA and Fusan (1 mM) did not affect sperm speed. 50% inhibition of sperm motility was calculated to be ~ 1.7 mM of Fusan concentration. In addition, two inhibitors, Chymostatin and Phosphoramidon were also tested with each experiment and had no effect on liquefaction or on sperm motility and speed. These results strongly suggest that plasmin may play an important role in the liquefaction process of human semen.

Key words: 6-amidino-2-naphtyl-6-guanidinobenzoate dihydrochloride (Fusan)/t-amino caproic acid/human semen liquefaction plasmin/proteinase inhibitor


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