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Hum. Reprod. Advance Access published online on March 11, 2004
Human Reproduction, doi:10.1093/humrep/deh187
© 2004 by European Society of Human Reproduction and Embryology
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Received November 25, 2003
Accepted January 9, 2004

Article

The recombinant subdomain IIIB of human serum albumin displays activity of gonadotrophin surge-attenuating factor

Sotiria Tavoulari 1, Stathis Frillingos 1*, Panayiota Karatza 1, Ioannis E. Messinis 2, and Konstantin Seferiadis 1

1 Laboratory of Biological Chemistry, University of Ioannina Medical School, Ioannina 45110, Greece
2 Department of Obstetrics and Gynecology, University of Thessalia Medical School, Larissa 41222, Greece

* To whom correspondence should be addressed. E-mail: efriligo{at}cc.uoi.gr.


  Abstract

BACKGROUND: Gonadotrophin surge-attenuating factor (GnSAF) is an as yet unidentified ovarian factor that acts on the pituitary to attenuate the pre-ovulatory LH surge. In a previous study, GnSAF bioactivity was proposed to derive, at least in part, from a C-terminal domain (95peptide) of human serum albumin (HSA). METHODS AND RESULTS: We employ here the expression-secretion system of Pichia pastoris to produce and assay selected recombinant polypeptides of HSA for GnSAF activity. We show that the C-terminal 95peptide of HSA (residues 490-585; subdomain IIIB) can be expressed from P.pastoris in secreted form and supernatants from clones expressing this polypeptide reduce the GnRH-induced LH secretion of primary rat pituitary cultures by 50-82%. When expressed in the same system, HSA domain III (residues 381-585) or full-length HSA (residues 1-585) are inactive. The bioactive subdomain IIIB is also separable from either domain III or full-length HSA on Blue Sepharose chromatography. CONCLUSIONS: Taken together, the findings highlight the putative importance of HSA subdomain IIIB as a GnSAF-bioactive entity and introduce a unique experimental tool to engineer this molecule for structure-function analysis.

Key words: Key words: albumin/GnRH/GnSAF/LH/Pichia pastoris


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