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Hum. Reprod. Advance Access published online on March 25, 2004
Human Reproduction, doi:10.1093/humrep/deh220
© 2004 by European Society of Human Reproduction and Embryology
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Received April 11, 2003
Revised December 1, 2003
Accepted February 18, 2004

Article

PCR-detected hepatitis C virus RNA associated with human zona-intact oocytes collected from infected women for ART

A. Papaxanthos-Roche 1*, P. Trimoulet 2, M. Commenges-Ducos 3, C. Hocké 4, H.J.A. Fleury 2, and G. Mayer 1

1 Laboratoire de Biologie de la Reproduction, Maternité Pellegrin, Centre Hospitalier Universitaire, Hôpital Pellegrin, Place Amélie Raba Léon, 33076 Bordeaux, France
2 Laboratoire de Virologie, Centre Hospitalier Universitaire, Hôpital Pellegrin, Place Amélie Raba Léon, 33076 Bordeaux, France
3 Service de Gynécologie-Obstétrique, Centre Hospitalier Universitaire, Hôpital Pellegrin, Place Amélie Raba Léon, 33076 Bordeaux, France
4 Service de Gynécologie, Hôpital Saint-André, 1, rue Jean Burguet, 33075 Bordeaux, France

* To whom correspondence should be addressed. E-mail: aline.papaxanthos{at}chu-bordeaux.fr.


  Abstract

BACKGROUND: The aim of this study was to investigate the susceptibility of human oocytes from hepatitis C virus (HCV) RNA-positive women to HCV contamination during assisted reproductive technology (ART). METHODS: A reverse transcriptase-PCR assay was used to test for the presence of HCV RNA associated with 24 unfertilized oocytes 48 h after follicular fluid aspiration in 10 IVF attempts (seven conventional IVF and three ICSI). Negative and positive controls (10 unfertilized oocytes from HCV-negative women and 20 unfertilized oocytes artificially contaminated with HCV RNA-positive plasma; HCV RNA was also quantified in plasma and follicular fluid) were included. RESULTS: HCV RNA was associated with 17/24 (70.8%) oocytes (6/7 after ICSI and 11/17 after conventional IVF) and was found in 19/20 (95%) follicular fluid samples. A weak correlation was found between plasma and follicular fluid HCV RNA loads (r = 0.73, P < 0.001). CONCLUSIONS: HCV associated with unfertilized oocytes surrounded by their intact zona pellucida from anti-HCV antibody-positive and viraemic women undergoing ART raises questions concerning the safe management of medically assisted procreation for these women and good practice of oocyte/embryo cryopreservation and donation.

Key words: Key words: hepatitis C virus/IVF/oocyte


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