Hum. Reprod. Advance Access published online on April 22, 2004
Human Reproduction, doi:10.1093/humrep/deh279
© 2004 by European Society of Human Reproduction and Embryology
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Prenatal Diagnosis Center, Department of Obstetrics and Gynecology, Cathay General Hospital, Taipei, Taiwan; Department of Obstetrics and Gynecology, College of Medicine, Taipei Medical University, Taiwan; Prenatal Diagnosis Center
* To whom correspondence should be addressed. E-mail: mstsai{at}ms1.cgh.org.tw.
BACKGROUND: The aim of this study was to isolate mesenchymal stem cells (MSCs) from amniotic fluid obtained by second-trimester amniocentesis. METHODS: A novel two-stage culture protocol for culturing MSCs was developed. Flow cytometry, RT-PCR and immunocytochemistry were used to analyse the phenotypic characteristics of the cultured MSCs. Von Kossa, Oil Red O and TuJ-1 stainings were used to assess the differentiation potentials of MSCs. RESULTS: Amniotic fluid-derived MSCs (AFMSCs) were successfully isolated, cultured and enriched without interfering with the routine process of fetal karyotyping. Flow cytometry analyses showed that they were positive for SH2, SH3, SH4, CD29, CD44 and HLA-ABC (MHC class I), low positive for CD90 and CD105, but negative for CD10, CD11b, CD14, CD34, CD117, HLA-DR, DP, DQ (MHC class II) and EMA. Importantly, a subpopulation of Oct-4-positive cells was detectable in our cultured AFMSCs. Under specific culture conditions, AFMSCs could be induced to differentiate into adipocytes, osteocytes and neuronal cells. CONCLUSIONS: We demonstrate that human multipotent MSCs are present in second-trimester amniotic fluid. Considering the great potential of cellular therapy using fetal stem cells and the feasibility of intrauterine fetal tissue engineering, amniotic fluid may provide an excellent alternative source for investigation of human MSCs. Key words:
Key words: amniotic fluid cells/mesenchymal stem cells/Oct-4 expression
Accepted March 29, 2004
Article
Isolation of human multipotent mesenchymal stem cells from second-trimester amniotic fluid using a novel two-stage culture protocol
2 Prenatal Diagnosis Center, Department of Obstetrics and Gynecology, Cathay General Hospital, Taipei, Taiwan
3 Bioresource Collection and Research Center, Food Industry Research and Development Institute, Hsinchu, Taiwan
Abstract 
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  G. V. Rosland, A. Svendsen, A. Torsvik, E. Sobala, E. McCormack, H. Immervoll, J. Mysliwietz, J.-C. Tonn, R. Goldbrunner, P. E. Lonning, et al. Long-term Cultures of Bone Marrow-Derived Human Mesenchymal Stem Cells Frequently Undergo Spontaneous Malignant Transformation Cancer Res., July 1, 2009; 69(13): 5331 - 5339. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-M. Zheng, H.-Y. Zhao, X.-E Zhao, F.-S. Quan, S. Hua, X.-Y. He, J. Liu, X.-N. He, and H. Lin Development of cloned embryos from porcine neural stem cells and amniotic fluid-derived stem cells transfected with enhanced green fluorescence protein gene Reproduction, May 1, 2009; 137(5): 793 - 801. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Chan, S. Kumar, and N. M. Fisk First trimester embryo-fetoscopic and ultrasound-guided fetal blood sampling for ex vivo viral transduction of cultured human fetal mesenchymal stem cells Hum. Reprod., November 1, 2008; 23(11): 2427 - 2437. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. H. Moon, J. R. Lee, B. C. Jee, C. S. Suh, S. H. Kim, H. J. Lim, and H. K. Kim Successful vitrification of human amnion-derived mesenchymal stem cells Hum. Reprod., August 1, 2008; 23(8): 1760 - 1770. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Ng, S. Boucher, S. Koh, K. S. R. Sastry, L. Chase, U. Lakshmipathy, C. Choong, Z. Yang, M. C. Vemuri, M. S. Rao, et al. PDGF, TGF-{beta}, and FGF signaling is important for differentiation and growth of mesenchymal stem cells (MSCs): transcriptional profiling can identify markers and signaling pathways important in differentiation of MSCs into adipogenic, chondrogenic, and osteogenic lineages Blood, July 15, 2008; 112(2): 295 - 307. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K.E. Schwab, P. Hutchinson, and C.E. Gargett Identification of surface markers for prospective isolation of human endometrial stromal colony-forming cells Hum. Reprod., April 1, 2008; 23(4): 934 - 943. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Pountos, E. Jones, C. Tzioupis, D. McGonagle, and P. V. Giannoudis Growing bone and cartilage: THE ROLE OF MESENCHYMAL STEM CELLS J Bone Joint Surg Br, April 1, 2006; 88-B(4): 421 - 426. [Full Text] [PDF]
|
|
|
|
 |
|
 M.-S. Tsai, S.-M. Hwang, Y.-L. Tsai, F.-C. Cheng, J.-L. Lee, and Y.-J. Chang Clonal Amniotic Fluid-Derived Stem Cells Express Characteristics of Both Mesenchymal and Neural Stem Cells Biol Reprod, March 1, 2006; 74(3): 545 - 551. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Leri, J. Kajstura, and P. Anversa Cardiac Stem Cells and Mechanisms of Myocardial Regeneration Physiol Rev, October 1, 2005; 85(4): 1373 - 1416. [Abstract] [Full Text] [PDF]
|
|