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Hum. Reprod. Advance Access published online on April 29, 2004
Human Reproduction, doi:10.1093/humrep/deh294
© 2004 by European Society of Human Reproduction and Embryology
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Received March 10, 2004
Accepted April 8, 2004

Article

Pregnancy following chemical activation of oocytes in a couple with repeated failure of fertilization using ICSI: Case report

Yoshihiko Murase 1*, Yasuyuki Araki 2, Satoshi Mizuno 3, Choji Kawaguchi 1, Mayumi Naito 1, Midori Yoshizawa 4, Yasuhisa Araki 2

1 Kanayama Lady’s Clinic, Meganeplaza Bldg. 6F, 1-201, Kanayama-cho, Atsuta-ku, Nagoya, 456-0002, Japan
2 The Institute for ARMT, 909-21, Ishii, Fujimi, Setagun, Gunma, 371-0105, Japan
3 IVF Osaka Clinic, 2-2-30, Nagatanaka, Higashi-Osaka-shi, Osaka, 550-0015, Japan
4 Department of Animal Breeding and Reproduction, Faculty of Agriculture, Utsunomiya University, 350, Mine-machi, Utsunomiya, Tochigi, 321-8505, Japan

* To whom correspondence should be addressed. E-mail: murase{at}klc.ne.jp.


  Abstract

We report our attempts to achieve a successful pregnancy outcome with calcium ionophore A23187 and puromycin oocyte activation using sperm from a normozoospermic husband of a patient with previous repeated failed fertilization following ICSI. Oocytes from the female partner of a couple with a 4 year history of unexplained primary infertility with repeated failed fertilization following ICSI were used. In the latest ICSI attempt, oocytes were activated by treatment with calcium ionophore (5 min) and puromycin (5 h), then cultured. In this cycle, assisted oocyte activation with calcium ionophore and puromycin after ICSI resulted in a satisfactory fertilization rate (8/12; 66.7%); in prior cycles only one of 71 oocytes (1.4%) was fertilized. The outcome was a Caesarean section delivery of a healthy male infant without congenital abnormalities at 41 weeks, 2 days of gestation. In conclusion, the use of calcium ionophore and puromycin for oocyte activation was found to be a useful method in a case of repeated failed fertilization after ICSI.

Key words: Key words: calcium ionophore/failed fertilization/ICSI/oocyte activation/puromycin


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