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Hum. Reprod. Advance Access published online on August 6, 2004
Human Reproduction, doi:10.1093/humrep/deh409
© 2004 by European Society of Human Reproduction and Embryology
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Received June 8, 2004
Accepted June 16, 2004

Article

Identification of viable embryos in IVF by non-invasive measurement of amino acid turnover

D.R. Brison 1*, F.D. Houghton 2, D. Falconer 3, S.A. Roberts 4, J. Hawkhead 2, P.G. Humpherson 2, B.A. Lieberman 5, H.J. Leese 2

1 Department of Reproductive Medicine, St Mary's Hospital, Manchester M13 OJH, UK
2 Department of Biology, University of York, P.O.Box 373, York Y010 5YW, UK
3 Manchester Fertility Services, Manchester BUPA Hospital, Russell House, Russell Road, Manchester M16 8AJ, UK
4 Biostatistics Group, School of Epidemiology and Health Sciences, University of Manchester, Manchester M13 9PT, UK
5 Department of Reproductive Medicine, St Mary's Hospital, Manchester M13 OJH, UK; Manchester Fertility Services, Manchester BUPA Hospital, Russell House, Russell Road, Manchester M16 8AJ, UK

* To whom correspondence should be addressed. E-mail: Daniel.Brison{at}man.ac.uk.


  Abstract

BACKGROUND: IVF is limited by low success rates and an unacceptably high multiple pregnancy rate. These outcomes would be improved significantly if a single embryo of high viability could be replaced in each treatment cycle, but widespread acceptance of such a policy is hindered by the lack of predictive factors for embryo selection. We have conducted a retrospective clinical study of a novel non-invasive method of embryo selection based on the depletion/appearance of amino acids in the culture medium. METHODS: Fifty-three cycles of IVF treatment using ICSI were studied. Embryos were cultured for 24 h in 4 µl drops of medium containing a physiological mixture of 18 amino acids. The spent medium was analysed for amino acid content by high performance liquid chromatography. RESULTS: The turnover of three amino acids, Asn, Gly and Leu, was significantly correlated with a clinical pregnancy and live birth. These correlations were independent of known predictors, such as female age, basal levels of FSH, embryo cell number and embryo morphological grade. CONCLUSIONS: Non-invasive assay of amino acid turnover has the potential to improve significantly the prospective selection of the most viable embryos, or single embryo, for replacement in an IVF cycle.

Keywords: amino acid; developmental potential; human preimplantation embryo selection; IVF.
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