Skip Navigation



Hum. Reprod. Advance Access published online on July 8, 2004
Human Reproduction, doi:10.1093/humrep/deh411
© 2004 by European Society of Human Reproduction and Embryology
This Article
Right arrow FREE Full Text (PDF ) Freely available
Right arrow All Versions of this Article:
19/10/2356    most recent
deh411v1
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Print, C.
Right arrow Articles by Smith, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Print, C.
Right arrow Articles by Smith, S.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Received November 5, 2003
Revised May 18, 2004
Accepted June 16, 2004

Article

Soluble factors from human endometrium promote angiogenesis and regulate the endothelial cell transcriptome

Cristin Print 1* *, Reija Valtola 1 *, Amanda Evans 1, Khashayar Lessan 1, Shazia Malik 2, Stephen Smith 2

1 Department of Pathology, Cambridge University, Tennis Court Road, Cambridge, CB2 1QP, UK
2 Department of Obstetrics and Gynaecology, The Rosie Hospital, Robinson Way, Cambridge CB2 2SW, UK

* To whom correspondence should be addressed. E-mail: cgp22{at}cam.ac.uk.


  Abstract

BACKGROUND: Angiogenesis and vascular remodeling play critical roles in the cyclical growth and regression of endometrium. They also appear to play roles in the pathogenesis of endometriosis. METHODS and RESULTS: Supernatants were collected from cultured endometrium isolated from women with and without endometriosis. These supernatants induced endothelial cell proliferation and angiogenesis in vitro. They contained vascular endothelial growth factor (VEGF)-A, and their proliferative effects on endothelial cells were partially abrogated by a blocking anti-VEGF-A antibody. Gene array analysis showed that culture supernatants from proliferative phase endometrium, and to a lesser extent secretory phase endometrium, induced significant changes in the transcriptome of endothelial cells. We could not detect any association between endometriosis and the ability of endometrial-derived soluble factors to promote angiogenesis or to regulate the endothelial transcriptome. In addition, we could not detect any association between endometriosis and the concentration of VEGF-A in supernatants from cultured endometrium or in menstrual effluent. CONCLUSIONS: We have shown that endometrium cultured in vitro produced soluble factors, including VEGF-A, that promoted angiogenesis. Proliferative phase endometrium promoted significant endothelial cell transcriptome changes that appear overall to be pro-angiogenic. These transcriptome changes provide insight into the dynamic control of vessel structure on which both eutopic endometrium and endometriotic lesions depend.

Keywords: endometrious; gene array; VEGF-A; transcriptome.

*These authors contributed equally to this work


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol Hum ReprodHome page
Z. Z. Zhao, D. R. Nyholt, S. Thomas, S. A. Treloar, and G. W. Montgomery
Polymorphisms in the vascular endothelial growth factor gene and the risk of familial endometriosis
Mol. Hum. Reprod., September 1, 2008; 14(9): 531 - 538.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
J. Gilabert-Estelles, L.A. Ramon, F. Espana, J. Gilabert, V. Vila, E. Reganon, R. Castello, M. Chirivella, and A. Estelles
Expression of angiogenic factors in endometriosis: relationship to fibrinolytic and metalloproteinase systems
Hum. Reprod., August 1, 2007; 22(8): 2120 - 2127.
[Abstract] [Full Text] [PDF]

Home page
 Hum Reprod UpdateHome page
T. Strowitzki, A. Germeyer, R. Popovici, and M. von Wolff
The human endometrium as a fertility-determining factor
Hum. Reprod. Update, September 1, 2006; 12(5): 617 - 630.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.