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Hum. Reprod. Advance Access published online on August 19, 2004
Human Reproduction, doi:10.1093/humrep/deh438
© 2004 by European Society of Human Reproduction and Embryology
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Received January 2, 2004
Revised February 12, 2004
Accepted April 2, 2004

Article

Morphologically normal spermatozoa of patients with secretory oligo-astheno-teratozoospermia have an increased aneuploidy rate

Nunziatina Burrello 1, Giuseppe Arcidiacono 2, Enzo Vicari 1, Paola Asero 1, Daniela Di Benedetto 1, Adele De Palma 2, Rosa Romeo 3, Rosario D'Agata 1, Aldo E. Calogero 1*

1 Section of Endocrinology, Andrology and Internal Medicine, Department of Biomedical Sciences, and Master in Andrological and Human Reproduction Sciences, Catania, Italy
2 Unit of Cardiology, Garibaldi Hospital, Catania, Italy
3 Department of Human Anatomy, University of Catania, Catania, Italy

* To whom correspondence should be addressed. E-mail: acaloger{at}unict.it.


  Abstract

BACKGROUND: Normal morphology is a major criterion for selecting spermatozoa to be injected. Given that teratozoospermia is one of the most critical parameters associated with sperm aneuploidy, the purpose of this study was to evaluate the aneuploidy rate of morphologically normal spermatozoa of patients with oligo-astheno-teratozoospermia (OAT). METHODS: Ten patients with secretory OAT and six age-matched normozoospermic men with a normal karyotype were enrolled. After assignment to normal or abnormal category, the location of each spermatozoon was recorded using an electronic microstage locator. Slides were then subjected to triple-colour fluorescence in situ hybridization for chromosomes X, Y and 12. RESULTS: OAT patients had a lower number of morphologically normal and abnormal spermatozoa carrying the X chromosome, compared with normozoospermic men. They also exhibited increased XY and XX disomy rates. Morphologically abnormal spermatozoa from normozoospermic men also had an increased XX disomy rate compared with normally shaped spermatozoa obtained from the same men. The total sperm aneuploidy rate of morphologically abnormal spermatozoa of normozoospermic men was 4.4-fold higher than that of spermatozoa with normal morphology. The total aneuploidy rates of spermatozoa with normal or abnormal head shape from OAT patients were similar to each other and to that of abnormally shaped spermatozoa from normozoospermic men, but they were higher than the rate found in normally shaped spermatozoa of normal men. CONCLUSIONS: Normally shaped spermatozoa of OAT patients have an increased aneuploidy rate.

Keywords: chromosomes 12; multicolour fluorescence in situ hybridization; oligo-astheno-teratozoospermia; sex chromosomes; aneuploidy; morphology.
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