Skip Navigation



Hum. Reprod. Advance Access published online on August 6, 2004
Human Reproduction, doi:10.1093/humrep/deh442
© 2004 by European Society of Human Reproduction and Embryology
This Article
Right arrow FREE Full Text (PDF ) Freely available
Right arrow All Versions of this Article:
19/10/2345    most recent
deh442v2
deh442v1
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Chen, Z.J.
Right arrow Articles by Feng, H.L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chen, Z.J.
Right arrow Articles by Feng, H.L.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Received January 2, 2004
Revised May 24, 2004
Accepted July 6, 2004

Article

Effects of sucrose concentration on the developmental potential of human frozen-thawed oocytes at different stages of maturity

Z.J. Chen 1, M. Li 2, Y. Li 2, L.X. Zhao 2, R. Tang 2, Y. Sheng 2, X. Gao 2, C.H. Chang 3, H.L. Feng 4*

1 Center for Reproductive Medicine, Shandong Provincial Hospital, Shandong University, Jinan 250021, China, Department of Medicine, Case Western Reserve University, Cleveland, Ohio 44106, USA
2 Center for Reproductive Medicine, Shandong Provincial Hospital, Shandong University, Jinan 250021, China
3 Department of Medicine, Case Western Reserve University, Cleveland, Ohio 44106, USA
4 Center for Human Reproduction, North Shore University Hospital, NYU School of Medicine, Manhasset, NY 11030, USA

* To whom correspondence should be addressed. E-mail: hfeng{at}nshs.edu.


  Abstract

BACKGROUD: Success of human oocyte cryopreservation depends on multiple cryobiological factors that could influence the developmental potential of the oocytes. The objective of this study was to examine the effects of different sucrose concentrations on the developmental potential of human frozen-thawed oocytes at different maturity stages. METHODS: A total of 355 oocytes collected from small follicles were randomly divided into three groups and two groups (B and C) were cryopreserved using slow-freezing method. Group A included 131 oocytes at different maturity stages without freezing. Another 119 oocytes in Group B were cryopreserved with 0.1 M sucrose and 105 oocytes in Group C with 0.2 M sucrose concentration. RESULTS: The post-thaw survival rate of the oocytes and the cleavage rate in Group C were significantly higher than that of Group B (P<0.05). For immature metaphase I (MI) stage oocytes, a significant difference was found in the maturation rate between Group C and Group B (P<0.05). The maturation rate for the GV oocytes in Groups A and C was significantly higher than Group B (P<0.01). CONCLUSIONS: The results suggested that sucrose concentration of 0.2 M in the cryoprotectant solution is more suitable for human oocyte cryopreservation.

Keywords: cryopreservation; embryo; human fertilization in vitro; ooctyes.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Hum Reprod UpdateHome page
D. A. Gook and D. H. Edgar
Human oocyte cryopreservation
Hum. Reprod. Update, November 1, 2007; 13(6): 591 - 605.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
P.E. Levi Setti, E. Albani, P.V. Novara, A. Cesana, and G. Morreale
Cryopreservation of supernumerary oocytes in IVF/ICSI cycles
Hum. Reprod., February 1, 2006; 21(2): 370 - 375.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
W.T. Zheng, G.L. Zhuang, C.Q. Zhou, C. Fang, J.P. Ou, T. Li, M.F. Zhang, and X.Y. Liang
Comparison of the survival of human biopsied embryos after cryopreservation with four different methods using non-transferable embryos
Hum. Reprod., June 1, 2005; 20(6): 1615 - 1618.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
S.J. Paynter, A. Borini, V. Bianchi, L. De Santis, C. Flamigni, and G. Coticchio
Volume changes of mature human oocytes on exposure to cryoprotectant solutions used in slow cooling procedures
Hum. Reprod., May 1, 2005; 20(5): 1194 - 1199.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
V. Bianchi, G. Coticchio, L. Fava, C. Flamigni, and A. Borini
Meiotic spindle imaging in human oocytes frozen with a slow freezing procedure involving high sucrose concentration
Hum. Reprod., April 1, 2005; 20(4): 1078 - 1083.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.