Hum. Reprod. Advance Access published online on August 27, 2004
Human Reproduction, doi:10.1093/humrep/deh444
© 2004 by European Society of Human Reproduction and Embryology
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1 Department of Environmental Health, Harvard School of Public Health, Simmons College, Boston, MA, USA
* To whom correspondence should be addressed. E-mail: rhauser{at}hohp.harvard.edu.
BACKGROUND: Members of the general population are exposed to non-persistent insecticides at low levels. The present study explored whether environmental exposures to carbaryl and chlorpyrifos are associated with DNA damage in human sperm. METHODS: Subjects (n=260) were recruited through a Massachusetts infertility clinic. Individual exposures were measured as spot urinary metabolite concentrations of chlorpyrifos [3,5,6-trichloro-2-pyridinol (TCPY)] and carbaryl [1-naphthol (1N)], adjusted using specific gravity. Sperm DNA integrity was assessed by neutral comet assay and reported as comet extent, percentage DNA in comet tail (Tail%) and tail distributed moment (TDM). RESULTS: A statistically significant increase in Tail% was found for an interquartile range (IQR) increase in both 1N [coefficient = 4.1; 95% confidence interval (CI) 1.9-6.3] and TCPY (2.8; 0.9-4.6), while a decrease in TDM was associated with IQR changes in 1N (-2.2; -4.9 to 0.5) and TCPY (-2.5; -4.7 to -0.2). A negative correlation between Tail% and TDM was present only when stratified by comet extent, suggesting that Tail% and TDM may measure different types of DNA damage within comet extent strata. CONCLUSIONS: Environmental exposure to carbaryl chlorpyrifos may be associated with increased DNA damage in human sperm, as indicated by a change in comet assay parameters.
Accepted July 9, 2004
Article
Urinary levels of insecticide metabolites and DNA damage in human sperm
2 Department of Bioengineering, University of Washington, Seattle, WA, USA
3 Department of Biostatistics, Harvard School of Public Health, Simmons College, Boston, MA, USA
4 Nursing Program, School for Health Studies, Simmons College, Boston, MA, USA
5 Centers for Disease Control and Prevention, Atlanta, GA, USA
6 Department of Environmental Health, Harvard School of Public Health, Simmons College, Boston, MA, USA; Vincent Memorial Obstetrics and Gynecology Service, Andrology Laboratory and In Vitro Fertilization Unit, Massachusetts General Hospital, Boston, MA, USA
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