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Hum. Reprod. Advance Access published online on December 2, 2004
Human Reproduction, doi:10.1093/humrep/deh636
© 2004 by European Society of Human Reproduction and Embryology
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Received November 26, 2003
Revised August 16, 2004
Accepted November 2, 2004

Article

Prediction of outcomes of assisted reproduction treatment using the calcium ionophore-induced acrosome reaction

Takafumi Katsuki 1, Tetsuaki Hara 1*, Katsunori Ueda 2, Junko Tanaka 3, and Koso Ohama 2

1 Department of Obstetrics and Gynecology, Infectious Disease Control and Prevention, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan
2 Department of Obstetrics, Hiroshima Prefectural Hospital, Hiroshima, Japan
3 Department of Epidemiology, Infectious Disease Control and Prevention, Graduate School of Biomedical Sciences, Hiroshima University, Hiroshima, Japan

* To whom correspondence should be addressed.
Tetsuaki Hara, E-mail: tetsuaki{at}hiroshima-u.ac.jp


  Abstract

BACKGROUND: Sperm concentration and motility are poor predictors of the outcome of intrauterine insemination (IUI), hysteroscopic intratubal insemination (HIT), or complete fertilization failure (CFF) in conventional IVF. We investigated whether the calcium ionophore-induced acrosome reaction (AR) constitutes an additional indicator of CFF and pregnancy that is independent of these semen parameters. METHODS: Infertile couples with no female factor (n=388) and women with tubal obstruction (n=32) were studied: IVF (n=133), ICSI (n=72), HIT (n=245) and IUI (n=61). The percentage of acrosome-reacted sperm in relation to viable sperm was calculated. Receiver operating characteristic curve and multiple logistic regression analyses were used to determine threshold values and the best predictor for CFF and pregnancy. RESULTS: Threshold values of AR for predicting CFF in IVF and pregnancy in IVF and HIT + IUI were 21, 26 and 22% respectively. These values were independent of the conventional semen analysis parameters. CFF was lower (2 versus 20%; P<0.01) and the pregnancy rate was higher (46 versus 24% P<0.05) for those with AR >21% in IVF. CFF and pregnancy rate in ICSI did not differ according to AR. Pregnancy rate was higher for those with an AR >22% for HIT + IUI (23 versus 11% P<0.01). CONCLUSIONS: Ionophore-induced AR appears to be a useful indicator in addition to routine semen analysis for selection of patients for treatment with appropriate assisted reproduction procedure.

Keywords: acrosome reaction; assisted reproductive technology outcomes; calcium ionophore; complete fertilization failure.
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