Skip Navigation



Hum. Reprod. Advance Access published online on February 10, 2005
Human Reproduction, doi:10.1093/humrep/deh756
This Article
Right arrow FREE Full Text (PDF ) Freely available
Right arrow All Versions of this Article:
20/5/1364    most recent
deh756v1
Right arrow Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Summers, M.C.
Right arrow Articles by Biggers, J.D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Summers, M.C.
Right arrow Articles by Biggers, J.D.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2005. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oupjournals.org
Received May 4, 2004
Revised October 18, 2004
Accepted December 14, 2004

Article

Mouse embryo development following IVF in media containing either l-glutamine or glycyl-l-glutamine

M.C. Summers 1, L.K. McGinnis 2, J.A. Lawitts 3, and J.D. Biggers 4*

1 Reproductive Science Center, One Forbes Road, Lexington, MA, 02421 and Department of Obstetrics and Gynecology, University of Massachusetts Medical School, Worcester, MA, 10655, USA
2 Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA, 02115, USA
3 Beth Israel Deaconess Hospital, Boston, MA 02115, USA
4 Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA, 02115, USA

* To whom correspondence should be addressed.
J.D. Biggers, E-mail: john_biggers{at}hms.harvard.edu


  Abstract

BACKGROUND: The development of the mouse zygote following fertilization in vitro in a KSOM-type medium containing either l-glutamine or glycyl-l-glutamine has been examined, and compared with the development of mouse zygotes produced by natural fertilization. METHODS: Mouse IVF, embryo culture and embryo transfer. RESULTS: Fertilization rates, development to the blastocyst stage, implantation rate, gross fetal development and fetal body weight are not different in a KSOM-type medium containing either l-glutamine or glycyl-l-glutamine. No evidence of abnormal fetal development, such as exencephaly, was observed. The replacement of l-glutamine with glycyl-l-glutamine favoured the development of relatively more inner cell mass cells than trophectoderm cells, and reduced the numbers of pyknotic and fragmented nuclei in the blastocysts that developed in vitro. CONCLUSIONS: There is no evidence that the presence of glutamine in the medium used for IVF influences significantly the subsequent development of the zygote. Replacing glutamine with glycyl-l-glutamine may be advantageous.

Keywords: exencephaly; glutamine; glycylglutamine; IVF.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Hum ReprodHome page
J. D. Biggers, L. McGinnis, and M. C. Summers
Reply: One-step versus two-step culture of mouse preimplantation embryos
Hum. Reprod., July 1, 2006; 21(7): 1936 - 1939.
[Full Text] [PDF]

Home page
 ReproductionHome page
H. Sagirkaya, M. Misirlioglu, A. Kaya, N. L First, J. J Parrish, and E. Memili
Developmental and molecular correlates of bovine preimplantation embryos.
Reproduction, May 1, 2006; 131(5): 895 - 904.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
J.D. Biggers, L.K. McGinnis, and J.A. Lawitts
One-step versus two-step culture of mouse preimplantation embryos: is there a difference?
Hum. Reprod., December 1, 2005; 20(12): 3376 - 3384.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.